Wisconsin Alumni Research Foundation

Andrew T Ulijasz

Richard David Vierstra

Bernard Weisblum

Robert M Corn

Katrina T Forest

Matthew G Erickson

Junrui Zhang

Emily A Smith

David Anstrom

Matthew J Wanat

Jeremiah R Wagner

Genetically-engineered cyanochrome fluorophore molecules (fluorophores) with increased fluorescence and with absorbing fluorescence in the blue and green (blue/green) portion of the light spectrum are provided. These fluorophores are derived from the domains of phytochromes, and in particular cyanobacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided.

Cyanochrome fluorophores

Genetically-engineered fluorophore molecules with increased fluorescence are provided. These fluorophores are derived from the domains of phytochromes, and in particular bacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided.

Phytochrome-based fluorophores

Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an &#x3c9;-modified alkanethiol monolayer to the substrate and then contacting the &#x3c9;-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.

Fusion protein arrays on metal substrates for surface plasmon resonance imaging

Disclosed herein are methods for exposing a cell to a peptide so as to inhibit binding in a two-component signal transduction system. Such methods can control the development of antibiotic resistance in pathogenic bacteria and other hosts. Peptides useful in such methods, and methods for identifying such peptides, are also disclosed.

Peptide ligands that mimic sensor kinases and inhibit response regulators

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Twickenham, United Kingdom

Andrew T Ulijasz