University of California

Andrew Smith

Miten Jain

David Bernick

Provided are methods of producing a nucleic acid complex. In certain aspects, the methods include combining a sample including ribosomal RNA (rRNA) and a probe complement oligonucleotide with an oligonucleotide probe. The oligonucleotide probe includes a 3' region complementary to a 3&#x2032; region of a rRNA, and a 5&#x2032; region complementary to the probe complement oligonucleotide. The combining is under conditions in which the 3&#x2032; region of the oligonucleotide probe hybridizes to the 3&#x2032; region of the rRNA and the 5&#x2032; region of the oligonucleotide probe hybridizes to the probe complement oligonucleotide, thereby producing a nucleic acid complex. In certain aspects, the methods find use in producing rRNA libraries that find use, e.g., in rRNA sequencing applications. Oligonucleotide probes, libraries thereof, compositions, and kits that find use, e.g., in practicing the methods of the present disclosure, are also provided.

Methods of producing ribosomal ribonucleic acid complexes

The invention also encompasses novel structures and methods comprising providing a molecular adapter for capture and manipulation of transfer RNA. The adaptor is bound to a tRNA molecule. The adaptor may be a cholesterol-linked DNA adapter oligonucleotide. The invention is useful in sequencing, identification, manipulation and modification of tRNA.

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Santa Cruz, CA, United States of America

Andrew Smith