Glaxosmithkline Intellectual Property Development Limited

Gilead Sciences, Inc.

Andrew S Thomson

Gregory Notte

Keshab Sarma

Lars V Heumann

Brandon Heath Brown

Mark Uden

Anna Chiu

Troy Evan Reynolds

Kent E Goklen

Nolan Griggs

William John Kenneth Lewis

Brenda J Burke Chan

Andre C Dumetz

Nicholas E Levy

Kayeleigh Farrow

Jessica Rachel Molek

Kenneth G Yancey

Hella Bosteels

Tao Wang

Richard Kucia-Tran

Shugui Chen

David Allen Siler

Stephan P Lathrop

The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support.

Methods for purifying antibodies

The present invention relates to variant antibodies and methods of generating said antibodies with a reduced level of binding to process impurities. In particular, the invention describes variant IgG4 antibodies which have been modified in the heavy chain constant region at any one or a combination of amino acids in the region between Kabat residues 203 and 256, wherein the variant IgG4 antibody has a reduced level of binding to host cell protein (HCP), compared to an unmodified IgG4 antibody. The invention also relates to compositions comprising said variant IgG4 antibodies.

Antibodies with reduced binding to process impurities

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Collegeville, PA, United States of America

Andrew S Thomson