Oregon State University

Andrew Robert Osborn

Taifo Mahmud

Alan Bakalinsky

Garrett Holzwarth

Disclosed is a synthetic gene cluster for producing gadusol derivatives, expression vectors and host cells containing the same, methods of producing gadusol derivatives, and compositions thereof. In an example, the synthetic gene cluster includes a valA nucleotide sequence capable of expressing ValA protein; a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein; a mysC nucleotide sequence capable of expressing a MysC protein; and a mysD nucleotide sequence capable of expressing a MysD protein. In this way, gadusol derivatives can be produced in amounts sufficient for use in a variety of applications.

Gadusol derivative production in bacteria

The present disclosure relates to engineered microorganisms capable of producing gadusol. The engineered microorganisms contain a nucleotide sequence encoding 2-epi-5-valione synthase (EEVS) and a nucleotide sequence encoding methyltransferase-oxidoreductase (MT-Ox). Methods of using the engineered microorganisms to produce gadusol, including the culturing of such microorganisms, are also described.

Growing community of inventors

Corvallis, OR, United States of America

Andrew Robert Osborn