Venturedyne, Ltd.

Andrew R Veldt

Clinton A Peterson

Richard L Walker

Kevin D Ewing

Darin E Immink

Syed M Jalal

Jack L Spurbeck

Daniel Dorn

A test chamber that is capable of operating in a mode where the temperature of the chamber is efficiently cooled without removing a substantial amount of moisture from the air. In one aspect, the test chamber includes a structure defining a work space having air, and a temperature control system (e.g., a refrigeration system having a compressor, a condenser, and an evaporator valve). The temperature control system includes a heat exchanger (e.g., an evaporator) positioned to communicate with the air in the work space, a source of cold fluid (e.g., a compressed, condensed, and throttled refrigerant) coupled to the heat exchanger, a source of hot fluid (e.g., compressed refrigerant gas) coupled to the heat exchanger, and a controller for controlling a mixture of cold fluid and hot fluid entering the heat exchanger (e.g., by adjusting a cold fluid valve and/or a hot fluid valve). In order to limit the loss of humidity caused by condensation on the heat exchanger, it is preferred that the controller is programmed such that the temperature of the mixture entering the heat exchanger is controlled to limit a temperature differential between the heat exchanger and the air in the work space.

Test chamber with temperature and humidity control

A thermal cycling system includes a structure defining a load space, a heating assembly positioned to heat to the load space, and a cooling assembly positioned to cool the load space. The cooling assembly includes a refrigeration circuit and a banking circuit. The refrigeration circuit has a compressor, a refrigeration condenser, a first refrigeration evaporator in direct thermal communication with the load space, a second refrigeration evaporator in parallel with the first evaporator, and primary refrigerant circulating throughout the refrigeration circuit. The banking circuit is gravity fed and includes a banking condenser, a banking evaporator positioned to cool the load space, and a banking refrigerant circulating throughout the banking circuit. The banking condenser includes a collection reservoir to store liquid banking refrigerant and is in thermal communication with the second refrigeration evaporator.

Refrigeration capacity banking for thermal cycling

The cytogenetic chamber includes a chamber enclosure, a drying cavity in the enclosure, a door and a hand-insertion port through the door. A slide-supported, fixative-treated cell (prepared outside the chamber or in situ in the chamber) is in the drying area and air flows through the entirety of the drying area at a substantially uniform (and relatively low) flow rate. The fixative dries at substantially the same drying rate, irrespective of the location of the slide in the drying area. The invention is particularly useful for chromosome spreading and results in a significantly-increased percentage of metaphases having metaphase areas in the optimal range of 2,500 to 4,500 squared microns.

Cytogenetic chamber

The cytogenetic chamber includes a chamber enclosure, a drying cavity in the enclosure, a door and a hand-insertion port through the door. A slide-supported, fixative-treated cell (prepared outside the chamber or in situ in the chamber) is in the drying area and air flows through the entirety of the drying area at a substantially uniform (and relatively low) flow rate. The fixative dries at substantially the same drying rate, irrespective of the location of the slide in the drying area. A related method is also disclosed. The invention is particularly useful for chromosome spreading and results in a significantly-increased percentage of metaphases having metaphase areas in the optimal range of 2,500 to 4,500 squared microns.

Growing community of inventors

Saugatuck, MI, United States of America

Andrew R Veldt