The Charles Stark Draper Laboratory, Inc.

Andrew P Magyar

Melissa M Sprachman

Kirsty A McFarland

Peter Cavanagh

Erin Rosenberger

Gregg E Favalora

Steven J Byrnes

Richard H Morrison, Jr

Ian Ward Frank

Kenneth T Kotz

Charles A Lissandrello

Miles Rogers

Kettner John Frederick Griswold, Jr

Ryan A Dubay

Nicole E Raustad

Amanda Nicole Billings-Siuti

Stacey Markovic

Haiyao Huang

Emma Vargo

Michael Springer

Ting Pang

Compositions and methods for optically-verified, sequence-controlled polymer synthesis are described.

Optically verified polymer synthesis

Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3' end of a growing single-stranded DNA polynucleotide.

Modified template-independent DNA polymerase

A parallelized chain-synthesizing technique includes capillary tubes, where each tube provides multiple locations or addresses where a specific arbitrary sequence for polymeric chains can be synthesized. An optical addressing system selectively delivers light to the locations to mediate or control reactions in the tubes.

Apparatus for high density information storage in molecular chains

Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.

Miniaturized DNA microarray for small-volume sample processing

Methods of detecting unknown genetic modifications in a DNA sample from an organism are disclosed.

Hybridization immunoprecipitation sequencing (HIP-SEQ)

Aspects of the invention are directed to chemical and biological molecule sensing devices, methods of fabricating the chemical sensor devices, and methods of using those devices to detect chemical and biological molecules. The chemical sensor device may comprise a chemically-sensitive vertical slit field effect transistor (VeSFET) with a chemical recognition element attached to a gate structure and/or a channel of the VeSFET. The recognition element may be capable of binding to a chemical of interest such that the binding of the chemical to the recognition element results in a modification of current flow of the VeSFET, resulting in a detectable signal. The chemical sensor device may further comprise an amplifier configured to receive the detectable signal and produce an amplified signal, and an analog-to-digital converter (ADC) configured to receive the amplified signal and to produce a digital signal that represents the amplified signal.

VeSFET chemical sensor and methods of use thereof

A composition for use in extending a molecular chain includes a carrier group and a payload group. The carrier group comprising a signaling group and a blocking group. A first bond bonds the payload group to the carrier group and a second bond bonds the signaling group to the blocking group. The first and second bonds are selectively cleavable such that the first bond is cleavable without cleaving the second bond. The payload group is to be attached to the molecular chain, while the blocking group is to block further attachments to the molecular chain once the payload group has been attached. The signaling group comprises a photon emitter that emits a signature photon in response to interrogation by an interrogatory photon. The carrier group transitions from a loaded state to an empty state upon cleavage of the first covalent bond.

Composition for use in molecular chain synthesis

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Arlington, MA, United States of America

Andrew P Magyar