Battelle Memorial Institute

Andrew P Bartko

Theodore J Ronningen

Ryan W Daly

Johnway Gao

Matthew S Young

Christopher Gordon Scheitlin

Kurt Blohm

David R Chase

Rodney S Black

Scott M Gibbons

James R Busch

Bradley B Ross

Brad Ross

Susana Lira-Gonzalez

Benjamin J Richetti

Karen Walczak

Nathan Roehr

Aaron M Deal

Theofore J Ronningen

A process for creating a stabilized diode laser device is disclosed, where the stabilized diode laser device includes a unibody mounting plate and several chambers aligned along a transmission axis. Various optic components are placed in the chambers, and based on a transmission through the chambers, the optic components are aligned and secured within the chambers.

Stabilized diode laser

An apparatus for extracting a material from a liquid includes a concentration stage having a filter, a first path from the filter, and a second path from the filter. Under this configuration, the concentration stage accepts an initial liquid volume. A first liquid not having material collected by the filter is passed along the first path, and concentrated liquid having material therein, which is entrapped by the filter, is directed to the second path. The apparatus also includes an aerosolizing stage coupled to the concentration stage that converts the concentrated liquid into an aerosol and a drying stage that dries the aerosol such that material extracted from the aerosol onto a material substrate.

Extraction of materials from liquids

A stabilized diode laser device is disclosed, which includes a unibody mounting plate that is mated mechanically to a thermoelectric cooler. The unibody mounting plate comprises chambers in which components, including a laser diode, are aligned and secured. A combination of the secured components within the unibody mounting plate, along with the thermoelectric cooler, provides stabilization of the laser diode.

A liquid transfer line receives a liquid having particles suspended therein and transfers the liquid to a capillary tube. A support tube secures the capillary tube therein. The support tube is substantially airtight except at a designated position. An optional securement secures the capillary tube within the support tube. A discharge end of the capillary tube is spaced from, and receded into, a discharge end of the support tube. A chuck is positioned below the support tube and is operable to open and to close to seal the designated position of the support tube. A sample substrate filters the liquid introduced through the liquid transfer line. Yet further, the sample preparation system comprises a pump that draws a vacuum that exhausts portions of the liquid that are not retained on the sample substrate.

Particle sample preparation with filtration

An apparatus for extracting a material from a liquid includes a concentration stage having a tangential flow filter, a first path from the tangential flow filter, and a second path from the tangential flow filter. Under this configuration, the concentration stage accepts an initial liquid volume. A first liquid not having material collected by the tangential flow filter is passed along the first path, and concentrated liquid having material therein, which is entrapped by the filter, is directed to the second path. The apparatus also includes an aerosolizing stage coupled to the concentration stage that converts the concentrated liquid into an aerosol and a drying stage that dries the aerosol such that material extracted from the aerosol onto a material substrate.

A Raman silent substrate includes a base and a biologically compatible material layer over the base. The biologically compatible material layer is Raman silent, provides a predetermined capture efficiency corresponding to a desired application, and supports biological culturing. In certain implementations, the base comprises a glass layer and an aluminum layer between the glass layer and the biologically compatible material layer.

Sample preparation for spectroscopy analysis

Detecting threat materials used in explosives is performed by receiving a sample, and selecting an area of interest on the sample suspected of containing a threat material indicative of an explosive. Detection also includes interrogating the area of interest with a Raman laser producing a Raman spectrum, and comparing an amplitude of a first spectral region in the Raman spectrum to a first predetermined threshold. Detection further includes performing a verification, if the determination indicates that the area of interest includes the threat material, by checking the interrogated area of interest for a secondary indicator of the presence of the threat material, by comparing an amplitude of a second spectral region in the Raman spectrum, which is different from the first spectral region in the Raman spectrum, to a second predetermined threshold, and activating an indicator if the verification indicates that the area of interest contains the threat material.

Explosives detection using optical spectroscopy

A small area electrostatic aerosol collector combines electrostatic collection of aerosol particles and electrohydrodynamic spraying of fluid so that a sample collected electrostatically can have fluid applied thereto. The fluid may assist with disaggregation and/or desalinization of biological material collected onto a sample substrate. A controller associated with the collector may control an electrostatic charge device and a spraying device such that the charge device and spraying device may operate in alternating fashion, or the charge device and spraying device may operate simultaneously. Further, mechanical systems are provided, for the disaggregation of particulate clusters collected onto a sample substrate.

Electrohydrodynamic spraying

An apparatus comprises: a microscope objective focused on a microscope field of view; a light source including a laser generating an astigmatic beam and optics configured to couple the astigmatic beam into the microscope objective to produce high aspect ratio illumination at the microscope field of view; and a data acquisition system configured to generate data pertaining to light emanating from the microscope field of view responsive to the high aspect ratio illumination. The apparatus may be a Raman spectroscopy system. The laser may be an edge emitting laser. The optics of the light source may include an aspherical lens arranged to compensate the astigmatism of the astigmatic beam. The optics of the light source may include a diffraction grating arranged respective to the laser to provide feedback reducing a spectral full width at half maximum (FWHM) of the astigmatic beam.

Line light source for Raman or other spectroscopic system

A method for determining whether a sample includes a nitrate-based explosive comprises receiving the sample and selecting an area of interest on the sample. Then, at least a portion of the area of interest is interrogated with an optical source to produce a spectrum with an amplitude. The amplitude of a first portion of the spectrum is compared to a first predetermined threshold to determine if the sample includes a nitrate. After determining that the sample includes a nitrate in the area of interest, the amplitude of a second portion of the spectrum is compared to a second predetermined threshold to determine if the sample includes a secondary indicator. Then, an indicator is activated based on the determinations.

A manufacturing method comprises collecting a sample from a cell culture used by a manufacturing application, and controlling a Raman spectrometer to collect a Raman spectrum of a targeted volume within the sample. The method further comprises obtaining reference spectra uniquely associated with a known cell line, which comprise at least two of: spectral measurements of mycoplasma by itself, a contaminated cell line, and a pure cell line. Moreover, the method comprises comparing the reference spectra to the collected spectrum, and identifying whether there is at least one unnatural molecular composition within the collected spectrum based upon the comparison of the reference spectra to the collected spectrum. An indication is provided as to whether mycoplasma is detected in the collected Raman spectrum where at least one unnatural molecular composition is identified within the collected spectrum, and the manufacturing application is stopped where mycoplasma is detected in the collected Raman spectrum.

Identification of mycoplasm contamination using Raman spectroscopy

Line light source for raman or other spectroscopic system

A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.

contamination of a known cell line is detected by collecting a Raman spectrum of a targeted volume within a sample, the targeted volume containing a known cell line of interest, obtaining a reference spectrum uniquely associated with the known cell line where the obtained reference spectrum is known to be free ofand comparing, using a processing device, the reference spectrum to the collected spectrum.is further detected by identifying whether there are unnatural molecular compositions within the collected spectrum based upon the comparison of the reference spectrum to the collected spectrum and providing an indication as to whetheris detected in the collected Raman spectrum based upon whether unnatural molecular compositions are identified within the collected spectrum.

Growing community of inventors

Columbus, OH, United States of America

Andrew P Bartko