Becton Dickinson and Company Limited

Andrew Kuhn

James G Nadeau

Tobin J Hellyer

Robert W Rosenstein

Keith Edward Thornton

Dolores M Berger

William A Nussbaumer

Sha-Sha Wang

Sha Sha Wang

Keith Thornton

Robert Rosenstein

A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

Immuno-amplification

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Baltimore, MD, United States of America

Andrew Kuhn