Perkinelmer Health Sciences, Inc.

Micronics, Inc.

Revvity Health Sciences, Inc.

Andrew Kolb

C Frederick Battrell

Matthew Scott Bragd

Carolina L Elmufdi

Isaac Sprague

Justin L Kay

Alan Keith Lofquist

Martha Stone

Alan K Lofquist

Megan Couch

Asa Bergdahl

Daniel Hoffman

Rafael Rivera

A specimen collection and delivery apparatus for collecting clinical specimens and delivery of samples thereof to, e.g., microfluidic testing devices for diagnostic analysis is disclosed. The specimen collection and delivery apparatus includes a closure housing for coupling with a sample tube at one end, an open luer for coupling with a complementary luer on a microfluidic device at the other end, and a filter matrix disposed in the interior. The filter matrix is air permeable and liquid impermeable at atmospheric pressure and prevents sample from flowing through the open luer end when coupled to a sample tube containing a specimen. Under vacuum, the liquid sample fraction of the specimen can be drawn through the filter matrix and into the microfluidic testing device through the luer connection for diagnostic analysis.

Specimen collection and delivery apparatus

A microfluidic mixing device comprising two bellows pumps (), microfluidic cartridges comprising the same and methods for use of the same are provided. The disclosed device enables efficient mixing of samples at the microfluidic scale. More particularly, the microfluidic mixing device comprises: a first bellows pump (); a second bellows pump (); a first microchannel fluidly interconnecting the first bellows pump () with a sample inlet and a reagent reservoir, wherein the first microchannel comprises a valve (V) interposed between the pump and the inlet, and a valve (V) interposed between the pump and the reservoir; a second microchannel fluidly interconnecting the first bellows pump () with the second bellows pump (), wherein the second micro channel comprises a valve (V) interposed between the first and second pump; a third microchannel fluidly interconnecting the first bellows pump () with the second bellows pump, wherein the third micro channel comprises a valve (V) interposed between the first and second pump; a first and second pneumatic member pneumatically connected to the first and second bellows pumps; wherein, the volume of the second bellows pump () is greater than the volume of the first bellows pump ().

Microfluidic mixing device and method

Microfluidic cartridges or devices for serum separation and blood cross-match analysis are provided. The devices may include a serum separation subcircuit alone or in combination with a solute mixing subcircuit. The serum separation subcircuit promotes on-cartridge clotting of a blood sample and manipulates the flow of the separated serum sample for subsequent cross-match analysis with a second blood sample, for example. The solute mixing subcircuit includes at least two intake channels, one for a whole blood sample from, for example, a blood donor and the other for the separated serum sample from, for example, a transfusion recipient. The solute mixing subcircuit further includes a serpentine mixing channel conjoined to a downstream channel. Under vacuum generated by a conjoined finger pump, the two input streams fill the serpentine mixing and downstream channels due to capillary action, enabling visualization of an agglutination reaction.

Microfluidic devices and methods for performing serum separation and blood cross-matching

Fluidic cartridges, and manufacture thereof, having a plurality of circuit element subtypes containing pneumatically operated diaphragm members, where the diaphragm materials are selected for yield point, chemical resistance, breathability and other properties individually according to the fluidic element subtype are provided. A process of in-situ edge-bonded decoupage for forming diaphragm members inside a cartridge, and fluidic circuits having diaphragm members as active and passive circuit elements, including pumps, valves, vents, waste receptacles, reagent reservoirs, and cuvettes with optical windows, where the material composition of each individual diaphragm member may be selected from an assortment of materials during manufacture are also provided.

Fluidic circuits and related manufacturing methods

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Seattle, WA, United States of America

Andrew Kolb