Butamax Advanced Biofuels LLC

Butamax (tm) Advanced Biofuels LLC

E.i. Dupont De Nemours and Company

The Goodyear & Tire Rubber Company

Danisco US Inc.

Andrew C Eliot

Vasantha Nagarajan

Lori Ann Maggio-Hall

Gail K Donaldson

Dennis Flint

Charles Edwin Nakamura

Michael G Bramucci

Caroline M Peres

Zachary Quinn Beck

Jean-Francois Tomb

Dmitrii V Vaviline

Qiong Cheng

Robert Donald Fallon

Joachim C Ritter

Sourav Kumar Sengupta

Luan Tao

Hasan Dindi

Paul Veikko Viitanen

Michael Charles Grady

Ranjan Patnaik

Anthony Arthur Gatenby

Ekaterini Korovessi

Kristin Ruebling-Jass

Lixuan Lisa Huang

Qi Chen

Lisa L Huang

Mehmedalija Jahic

John S Chapman

John Joseph Hagedorn

Vasantha Nagajaran

Paulv Viitanen

The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.

Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

Fermentive production of four carbon alcohols

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of

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Disrupting expression of a protein encoded by the region of thegenome called ZMO0353 in the genomic sequence of the ZM4 strain was found to improve the use of xylose in a recombinant xylose utilizingcell. In addition, utilization of both xylose and arabinose was improved in a xylose and arabinose utilizingcell with this disruption, and increased ethanol production was achieved.

Enhancing D-xylose and L-arabinose utilization in Zymomonas cells

Method for the production of isobutanol by recombinant yeast

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.

Recombinant host cell comprising a diol dehydratase

Production of isobutanol by a microorganism with enhanced acetolactate synthase activity

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Wilmington, DE, United States of America

Andrew C Eliot