Third Wave Technologies, Inc.

Andrew A Lukowiak

Jeff G Hall

Victor I Lyamichev

Bruce P Neri

Hatim T Allawi

Peggy S Eis

Michael W Kaiser

Sarah Olson

Marilyn C Olson-Munoz

LuAnne Chehak

Hon Ip

Michelle Curtis

Christian Tor Bartholomay

Tsetska Y Takova

James J Schaefer

Kevin L Vedvik

WuPo Ma

Lin Ji

Robert W Kwiatkowski, Jr

Zbigniev Skrzypczynski

Natalie E Lyamicheva

Lisa C Thompson

Brad T Argue

Patrick Peterson

Natalie E Lyamichev

Sarah M Olson

Wupo Ma

Hon S Ip

Hatim Taysir Allawi

Luanne Chehak

Michelle L Curtis

Robert W Kwiatkowski

Zbigniew Skrzypczynski

Nancy Jarvis

David Kurensky

Natalie Lyamichev

Bruce N Neri

Jr Robert W Kwiatkowski

The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3' hairpin structures, 5&#x2032; hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5&#x2032; or 3&#x2032; ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.

Snap-back primers and detectable hairpin structures

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

RNA detection assays

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.

Amplification methods and compositions

The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5' nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Methods and compositions for detecting target sequences

Detection of RNA Sequences

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Stoughton, WI, United States of America

Andrew A Lukowiak