National Institutes of Health, a Component of the US Dept. of Health & Human Services

Andrei P Pomerantsev

Stephen H Leppla

The invention relates to a() in which more than one secreted protease is inactivated by genetic modification. Such a protease-deficienthas an improved ability to produce recombinant secreted proteins compared to other bacteria, particularly other. Improvements include production of intact (i.e., mature full-length) proteins, often at high yield. The disclosure provides athat comprises a genetic modification that inactivates a protease of the M4 family of metalloproteases and a genetic modification that inactivates a protease of the M6 family of metalloproteases. Also provided is a modifiedcomprising such genetic modification transformed with a recombinant molecule encoding a product, as well as methods to prepare and use such

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Bethesda, MD, United States of America

Andrei P Pomerantsev