Danisco US Inc.

The Goodyear & Tire Rubber Company

Dupont Nutrition Biosciences Aps

Danisco A/s

Danisco US Inc., Genencor Division

Andrei Miasnikov

Marguerite A Cervin

Joseph Charles McAuliffe

Gregory Marshall Whited

Fernando Valle

Frank James Feher

Aaron Scott Puhala

Gopal K Chotani

Karl John Sanford

Caroline M Peres

Richard La Duca

Birgitta Leuthner

Oliver Kensch

Ulrich Kettling

Richard Ray Bott

Jørn Borch Søe

Jørn Dalgaard Mikkelsen

Carol Marie Fioresi

Vijay Kumar

Andre Koltermann

Virve Pitkanen

Michael C Ward

James T Kellis, Jr

Derek H Wells

Walter Weyler

Christopher Lee Rife

Michael D Ward

Karsten Matthias Kragh

Shukun Yu

Mira Povelainen

Ilana S Aldor

Steven Sungin Kim

Klaus Pellangahr

Jens Frisbæk Sørensen

Jeffrey W Munos

Luis Fernando Romero Millan

Steve Sungjin Kim

Thomas Eisele

Michael W Schelle

Peter Edvard Degn

Heli Putaala

Luke Barnard

Klaus Pellengahr

Svend Haaning

Ernst Meinjohanns

Steffen Yde Bak

Stepan Shipovskov

Päivi Nurminen

Maria Ma

Joseph C Mcauliffe

Steve Kim

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Compositions and methods for producing isoprene

Described are methods for increasing the expression of a transgene in eukaryotic cells by reducing RNA interference (RNAi), and variant cells produce by the method. The methods and variant cells are useful, for example, for the efficient production of therapeutic and industrial polypeptides in eukaryotic cells.

Increasing the expression of a transgene in eukaryotic cells by reducing RNA interference

A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.

Altered host cell pathway for improved ethanol production

Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, andhost cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.

Thermostable trichoderma cellulase

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22%.

Polymers of isoprene from renewable resources

The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding tophytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.

Mutant citrobacter freundii phytase polypeptide

The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

Variant lipolytic enzymes and methods for expression thereof

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22&#x2030;.

Polymers of isropene from renewable resources

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

Isoprene synthase variants for improved microbial production of isoprene

The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera:andin various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable fromspecies.

Lipolytic enzyme: uses thereof in the food industry

Lipolytic enzyme uses thereof in the food industry

The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.

Method for introducing nucleic acids into fungal cells

Growing community of inventors

Mountain View, CA, United States of America

Andrei Miasnikov