Sharp Laboratories of America, Inc

Andrei L Gindilis

Paul John Schuele

John Hartzell

Pooran Chandra Joshi

Kevin Robert Schwarzkopf

Embodiments of the present invention are directed to efficient sensors for detecting the presence and concentration of one or more particular target molecules in solutions, air or other gasses, or otherwise present in an environment or sample. Various embodiments of the present invention provide relatively large binding constants or, equivalently, relatively small dissociation constants for binding of target biopolymers or other target molecules with complementary probes bound to, or associated with, a sensor substrate. In addition, various embodiments of the present invention increase the area of the surface of the sensor affected by binding of a target biopolymer or other target molecules to the sensor which, in turn, produces a stronger signal and greater signal-to-noise ratio for a given number or concentration of bound target biopolymers or other target molecules. In certain embodiments of the present invention, large binding constants, large signal-to-noise ratios, and strong signals are achieved by including probe molecules on the surface of the sensor directed to multiple binding sites within a target biopolymer or other target molecules, so that each bound target biopolymer or other target molecule is bound to, or associated with multiple probes.

Efficient sensor for detecting and determining the concentrations of targets

A system and method are provided for selectively functionalizing a transducer microarray. The method provides a microarray including a field of transducers exposed to a shared local environment. A difference in the pH associated with the transducers is created. As a result, functional molecules are selectively bound to transducers in response to the pH associated with the transducers. In one aspect, the micro-array also provides a field of transducer pH-generating electrodes, one pH-generating electrode for each transducer, and a counter electrode. The difference in pH associated with the transducers is created by selectively applying a voltage potential between pH-generating electrodes and the counter electrode, to create a difference of pH in regions adjacent to the transducers.

Selectively functionized transducer microarray

A method of performing a fluid-material assay employing a device including at least one active pixel having a sensor with an assay site functionalized for selected fluid-assay material. The method includes exposing the pixel's sensor assay site to such material, and in conjunction with such exposing, and employing the active nature of the pixel, remotely requesting from the pixel's sensor assay site an assay-result output report. The method further includes, in relation to the employing step, creating, relative to the sensor's assay site in the at least one pixel, a predetermined, pixel-specific electromagnetic field environment.

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Vancouver, WA, United States of America

Andrei L Gindilis