University of Chicago

Andrei D Mirzabekov

Gennadiy M Yershov

Alexander I Belgovskiy

Alexander Perov

Sergei Bavykin

Dmitry Yuryevich Guschin

Sergei Tillib

Vladimir M Zakhariev

Vladimir Mikhailovich

Edouard Timofeev

Aleksei Drobyshev

Alexander Fotin

Vadim Vasiliskov

Yuri Lysov

Valentine Chik

James P Akowski

Boris Strizhkov

Alexei Sharonov

A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.

Column device for isolation and labeling of nucleic acids

A device for producing microscopic arrays of molecules is provided, the device comprising a plurality of inverted cavities containing solutions, a substrate adapted to be received by the cavities for extracting the solutions, a substrate for depositing the extracted solutions onto a location on a matrix; and a quality control monitoring system for verifying that the solutions are deposited onto the location on the matrix. A process for producing an array of molecules also is provided, the process comprising providing a plurality of inverted solution cavities, wherein each cavity contains a solution; extracting each solution from its respective inverted cavity; loading each solution at a predetermined position in an array; and verifying that each solution is loaded onto its respective position in the array.

Device for producing microscopic arrays of molecules, a method for producing microscopic arrays of molecules

A method for making polymerized molecules is provided whereby a solution containing monomer is contacted to a solid substrate so as to form discrete accumulations of the monomer on the substrate; and the accumulations are contacted with a polymerizing agent, wherein the agent is dispersed in a vehicle which prevents cross contamination of the accumulations.

Method of fabrication of microarray of gel-immobilized compounds on a chip by copolymerization

The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.

PCR amplification on microarrays of gel immobilized oligonucleotides

This invention relates to using customized oligonucleotide microchips as biosensors for the detection and identification of nucleic acids specific for different genes, organisms and/or individuals in the environment, in food and in biological samples. The microchips are designed to convert multiple bits of genetic information into simpler patterns of signals that are interpreted as a unit. Because of an improved method of hybridizing oligonucleotides from samples to microchips, microchips are reusable and transportable. For field study, portable laser or bar code scanners are suitable.

Customized oligonucleotide microchips that convert multiple genetic information to simple patterns, are portable and reusable

A portable biochip scanner device used to detect and acquire fluorescence signal data from biological microchips (biochips) is provided. The portable biochip scanner device employs a laser for emitting an excitation beam. An optical fiber delivers the laser beam to a portable biochip scanner. A lens collimates the laser beam, the collimated laser beam is deflected by a dichroic mirror and focused by an objective lens onto a biochip. The fluorescence light from the biochip is collected and collimated by the objective lens. The fluorescence light is delivered to a photomultiplier tube (PMT) via an emission filter and a focusing lens. The focusing lens focuses the fluorescence light into a pinhole. A signal output of the PMT is processed and displayed.

Portable biochip scanner device

A biochip scanner device used to detect and acquire fluorescence signal data from biological microchips or biochips and method of use are provided. The biochip scanner device includes a laser for emitting a laser beam. A modulator, such as an optical chopper modulates the laser beam. A scanning head receives the modulated laser beam and a scanning mechanics coupled to the scanning head moves the scanning head relative to the biochip. An optical fiber delivers the modulated laser beam to the scanning head. The scanning head collects the fluorescence light from the biochip, launches it into the same optical fiber, which delivers the fluorescence into a photodetector, such as a photodiode. The biochip scanner device is used in a row scanning method to scan selected rows of the biochip with the laser beam size matching the size of the immobilization site.

Growing community of inventors

Darien, IL, United States of America

Andrei D Mirzabekov