Arcturus Therapeutics, Inc.

Andreas Wagner

Joseph E Payne

Padmanabh Chivukula

Priya Prakash Karmali

Yanjie Bao

Michael Figa

Scott A Roberts

A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1tube having an inner diameter (ID) of between about 0.1″ and 0.132″; b) flowing an ethanol solution comprising lipids through a 2tube having an ID of between about 0.005″ and 0.02″ at one third the flow rate of the aqueous solution through the 1tube, wherein the lipids comprise a cationic lipid; and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2tube perpendicularly joined to the 1tube; wherein the mixing produces an output solution flowing in the 1tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.

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Klosterneuburg, Austria

Andreas Wagner