Genomatica, Inc.

Reg Life Sciences, LLC

Ls9, Inc.

Kosan Biosciences Incorporated

Andreas W Schirmer

Mathew A Rude

Zhihao Hu

Shane A Brubaker

Baolong Zhu

Stephen B Del Cardayre

Haibo Wang

Cindy Chang

Na Trinh

Emanuela E Popova

Jacob Gano

Derek L Greenfield

Elizabeth J Clarke

Noah Helman

George M Church

Daniel T Chu

David Arthur Berry

Chaitan Khosla

Jay D Keasling

Daniel V Santi

Robert G McDaniel

Behnaz Behrouzian

Donald Eugene Trimbur

Maria Fardis

Louis G Hom

Murtaza F Alibhai

Vikranth Arlagadda

Ralph C Reid

Christopher Reeves

Myong K Ko

Kevin Holden

Alma Itzel Ramos-Solis

Lisa C Friedman

Grace J Lee

Jessica Winger

Andreas Rascher

Richard C Hutchinson

Chris Somerville

Eli S Groban

Tarah S Baron

Bernardo M Da Costa

John R Haliburton

Kseniya Zakharyevich

Katherine Ann Murphy

Erin Frances Perry

Elizabeth Clarke

Stephen B Del Cardayré

Chris Sommerville

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

Production of non-native monounsaturated fatty acids in bacteria

The present disclosure relates to chemo-enzymatic processes for the preparation of lactones (including, e.g., macrolactones, γ-lactones, and δ-lactones) and/or macrocyclic ketones, which are compounds of industrial value, for example, for use as fragrance ingredients. The chemo-enzymatic processes combine the in vivo microbial production of fatty acid derivatives and the subsequent ex vivo synthetic transformation of the fatty acid derivatives to provide the lactones and macrocyclic ketones.

Semisynthetic routes to organic compounds

The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

Omega-hydroxylase-related fusion polypeptide variants with improved properties

The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

Acyl-ACP reductase with improved properties

The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

Methods of producing omega-hydroxylated fatty acid derivatives

Omega-hydroxylase-related fusion polypeptides with improved properties

The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

Microbial production of fatty diols

Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.

Methods and compositions for producing hydrocarbons

The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.

Acetyl-CoA carboxylase variants

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

Production of fatty acid derivatives

The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.

Chemo-enzymatic process

Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.

Production of fatty acids and derivatives thereof

Growing community of inventors

South San Francisco, CA, United States of America

Andreas W Schirmer