Basf Aktiengesellschaft

Evonik Degussa Gmbh

Technische Universitat Dortmund

Technische Universitaet Dortmund

Andreas Schmid

Thomas Haas

Bernhard Hauer

Thomas Zelinski

Lars Mathias Blank

Bruno Buehler

Bruno Bühler

Harald Haeger

Steffen Schaffer

Martin Roos

Hans-Georg Joerg Hennemann

Andreas Karau

Thorsten Eggert

Steffen Howitz

Volker Sieber

Harald Häger

Peter Welters

Markus Pötter

Katrin Grammann

Guido Jach

Gabriele Sadowski

Christoph Brandenbusch

Katrin Schullehner

Mattijs Kamiel Julsing

Heike Steckhan, Legal Representative

Antje Steckhan, Legal Representative

Uwe Steckhan, Legal Representative

Christiane Karla Steckhan, Legal Representative

Frank Hollmann

Bernhard Witholt

Michael Goernert

Andreas Mueller

Bernd Lalla

Sjef Cornelissen

Manfred Schrewe

Frederik Sven Ole Fritzsch

Andreas MÜLLER

The invention relates to a method for the separation of a polarizable bioparticle comprising the steps: a) dielectrophoretic preseparation of a polarizable bioparticle from a suspension of bioparticles; b) fluidic separation of the selected bioparticle by fixing the bioparticle in a dielectrophoretic field cage and circulating fluid around the bioparticle; c) transferring the separated bioparticle from the dielectrophoretic field cage to a culture chamber; d) dielectrophoretic fixing of the separated bioparticle in the culture chamber and study, observation, manipulation and/or culturing of the separated bioparticle. The invention further relates to a microfluidic system and use thereof.

Method for the separation of polarizable bioparticles

The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.

Biocatalytic oxidation process with AlkL gene product

The present invention relates to a cell, which has been genetically modified relative to its wild type, so that in comparison with its wild type it is able to produce more &#x3c9;-aminocarboxylic acids, more &#x3c9;-aminocarboxylic acid esters or more lactams derived from &#x3c9;-aminocarboxylic acids, starting from carboxylic acids or carboxylic acid esters. Furthermore, the present invention relates to a method for the production of a genetically modified cell, the cells obtainable by this method, a method for the production of &#x3c9;-aminocarboxylic acids, of &#x3c9;-aminocarboxylic acid esters or of lactams derived from &#x3c9;-aminocarboxylic acids, the &#x3c9;-aminocarboxylic acids, &#x3c9;-aminocarboxylic acid esters or lactams derived from &#x3c9;-aminocarboxylic acids obtainable by this method, a method for the production of polyamides based on &#x3c9;-aminocarboxylic acids or based on lactams and the polyamides obtainable by this method.

&#x3c9;-Aminocarboxylic acids, &#x3c9;-aminocarboxylic acid esters, or recombinant cells which produce lactams thereof

The method separates emulsions derived from whole-cell biotransformations, including stable emulsions derived from typical biocatalytic two-phase processes that result with such a biotransformation. A supercritical extraction to obtain the valuable product can follow directly, because of the use of compressed or supercritical gas as the separation agent. It is unimportant whether the valuable product is present in the aqueous or the organic phase. Recycling of the organic phase is possible, since the surfactant cell components decisively responsible for the formation of the stable emulsion can be separated off via sedimentation, because of the treatment. The achieved separation remains in existence even after the gas has gassed out, so that aside from extraction, other methods for product isolation can also follow, if necessary.

Method for processing coalescence-inhibited emulsions from whole-cell biotransformations with compressed or supercritical gases

The invention relates to a process for the biocatalytic oxidation of aromatic compounds using recombinant xylene monooxygenase-expressing microorganisms in a biphasic reaction medium.

Method for the oxidation of aromatic compounds

A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NADformed by reductive cleavage of oxygen is electrochemically reduced, is described.

Method comprising the indirect electrochemical regeneration of NAD(P)H

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Dortmund, Germany

Andreas Schmid