Bayer Healthcare Aktiengesellschaft

Bayer Pharma Aktiengesellschaft

Bayer Aktiengesellschaft

Bayer Schering Pharma Ag

Triton Technology, Inc.

Andreas Schade

Wolfgang Paffhausen

Delf Schmidt

Martin Bechem

Thoams Krahn

Thomas Krahn

Nils Burkhardt

Klaus Ochmann

Bernd Kalthof

Michael Harnau

Karl-Hermann Koeching

Georg Schmidt

Mike Küster

Linn Schneider

Rainer Gross

Gerd Heusch

A dispensing device includes a housing having at least one pressure chamber, having a supply opening for the supply of liquid into the pressure chamber and having a multiplicity of conduits between the pressure chamber and an external side of the housing, there being situated in each of the conduits a tube, the first end of which protrudes into the pressure chamber and the second end of which protrudes out of the housing on the external side.

Metering device

A method and a system for rapid kinetic fluorescence measurement in high-throughput screening with high time-resolution includes providing a microtiter plate having a transparent base and multiple assay wells, a dispensing system arranged above the microtiter plate for simultaneously adding liquid into multiple assay wells, an illumination system beneath the microtiter plate for illuminating the multiple assay wells simultaneously through the transparent base, a detection system for detecting electromagnetic radiation from the multiple assay wells simultaneously through the transparent base, adding 0.3-300 &#x3bc;l of liquid per assay well from the dispensing system into the multiple assay wells, illuminating the multiple assay wells simultaneously through the transparent base using the illumination system before or from the time point of the start of the addition, detecting the electromagnetic radiation from the multiple assay wells simultaneously at a time interval of 1-1000 ms between individual measurement points.

Method and system for taking measurements in a high-throughput screening with high time resolution

A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.

Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays

In a process for the quantitative optical analysis of fluorescently labelled biological cells, a cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dye. The sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dye, which absorbs the excitation lightfor the fluorescent dyeand/or its emission light, is added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottom. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatant. Instead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom

Masking background fluorescence and luminescence in the optical analysis of biomedical assays

In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.

In a process for the quantitative optical analysis of fluorescently labelled biological cellsa cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dyeThe sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dyewhich absorbs the excitation lightfor the fluorescent dyeand/or its emission lightis added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottomThis process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatantInstead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom

Masking background flourescence and luminescence in optical analysis of biomedical assays

In a process for the quantitative optical analysis of fluorescently labelled biological cells , a cell layer on a transparent support at the bottom of a reaction vessel is in contact with a solution containing the fluorescent dye . The sensitivity of analytical detection can be considerably improved if to the fluorescent dye already present in addition a masking dye , which absorbs the excitation light for the fluorescent dye and/or its emission light , is added to the solution and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to the cell layer at the bottom . The separating layer must be composed such that it has a high power of reflection for the luminescent light . Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dye which absorbs the excitation light for the fluorescent dye and/or its emission light or the luminescent light is added to the supernatant . Instead of the masking dye in the solution or optionally as an additional measure, a separating layer permeable to the solution and absorbing and/or reflecting the excitation light and/or the emission light or the luminescent light can be applied to the cell or receptor layer at the bottom

Masking background fluorescence and luminescence in optical analysis of biomedical assays

An improved process for the measurement of blood circulation by means of colored microspheres is presented, which process replaces the previous counting procedure when using colored microspheres by a rapidly reproducible measurement method. This avoids the complicated and expensive use of radioactively labelled microspheres. At the same time, a coloring process for the preparation of the microspheres is presented which is distinguished by homogeneous and intensive coloration of the microspheres.

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Essen, Germany

Andreas Schade