Paradigm Genetics, Inc.

Other

Andreas S Kloti

Douglas C Boyes

Carol M Hamilton

Keith R Davis

Neil E Hoffman

Robert Ascenzi

J&ouml;rn G&ouml;rlach

Jorn Gorlach

John W Rice

Sandy J Stewart

Adel Zayed

Jeffrey P Woessner

Beth Lanning

Ruediger Hain

William Frank Martin

John M Crawford, Jr

Marco Busch

Klaus-Guenther Tietjen

Novel protein useful as a selectable marker resistant to the antibiotic nourseothricin and corresponding polynucleotides for insertion of genes and other genetic material into a variety of organisms, including plants are described.

Selectable marker in plants

Morphological markers are used in a method of visually identifying plants transformed with a nucleotide sequence (e.g., a heterologous gene). The nucleotide sequence is transformed into a plant that exhibits an abnormal phenotype for a morphological marker. If the transformation of the plant is successful, the progeny of the transformed plant will exhibit a normal phenotype. In a preferred embodiment, the plant is Arabidopsis and the morphological marker is Gl1, which is associated with trichome production on plant leaves. The method is also useful for identifying plants that are homozygous for the transformed gene, and for identifying transformants in the T2 generation that are true crosses, rather than self-crosses.

Methods for identifying transgenic plants using morphological markers

Methods for the identification of modulators of magnesium chelatase expression or activity in plants

A synthetic nucleotide sequence encodes the LexA DNA binding domain, the nucleotide sequence having been modified to bring the codon usage in conformity with the preferred codon usage of . The preferred sequence of the gene is provided as SEQ ID NO:1. DNA constructs, transformed host cells and transgenic plants comprising the synthetic nucleotide sequence are also provided.

Modified nucleotide sequence encoding a LexA DNA binding domain optimized for arabidopsis species

The invention relates to plants, seeds and DNA constructs containing DNA that is either SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, or the complementary or double stranded sequence thereof, as well as a method for their preparation.

Modified minimal promoters

This invention relates to plants, seeds and DNA constructs containing DNA that is either SEQ ID NO:10, SEQ ID NO:11, or SEQ ID NO:20, or the complementary or double stranded sequence thereof, as well as a method for their preparation.

Modified full length promoters

The invention is directed to methods and compositions for the determination of deoxyxylulose 5-phosphate synthase (DXPS) activity. The methods and compositions of the invention are amenable to high-throughput screening assays for the identification of inhibitors and enhancers of DXPS activity. Such compounds have use in the modulation of plant and microbial growth. The compositions of the invention are DXPS fragments and chimeric polypeptides that have increased solubility as compared to the wild-type DXPS polypeptide. These DXPS fragments and chimeric polypeptides can be recombinantly expressed and purified in quantities suitable for high-throughput screening assays. The assays of the invention are based on the detection of substrates of DXPS that remain after a DXPS reaction.

Methods for determining deoxyxylulose 5-phosphate synthase activity

The invention relates to DNA which encodes Arabidopsis 1-deoxy-xylulose-5-phosphate reductoisomerase and to a method of identifying modulators of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase activity.

Method of determining activity of 1-deoxy-D-xylulose-5-phosphate reductoisomerase and 1-deoxy-D-xylulose-5-phosphate synthase

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Durham, NC, United States of America

Andreas S Kloti