Roche Diagnostics Operations, Inc.

Andreas Leinenbach

Uwe Kobold

Martin Rempt

Dieter Heindl

Hans-Peter Josel

Christoph Seidel

Giuseppe Prencipe

Simon Ferdinand Loibl

Silvia Baecher

Jelena Milic

Nicole Pirkl

Anna-Skrollan Geiermann

Hartmut Duefel

Thomas Carell

Robert Lang

Robert Hahn

Aart Pieter Van Doorn

Harmut Duefel

Markus Muller

Sarah Schiffers

Rene Rahimoff

Olesea Kosmatchev

Indranil Mitra

Angie Kirchner

Holger Busskamp

Toni Pfaffeneder

The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

Reagent for mass spectrometry

The present invention refers to a method for determining intact glycated hemoglobin A (HbA1c) by mass spectrometry (MS), a kit and a diagnostic system adapted for performing the method.

Automatic sample workflow for LC-MS based HbA1c measurement on intact protein level

A liquid chromatographic (LC) system is herein disclosed, the LC system comprising at least one fluidic stream comprising at least one HPLC column, a downstream valve connected to the at least one fluidic stream and connectable to a detector via a valve-to-detector conduit, wherein the at least one fluidic stream is connectable to the valve-to-detector conduit via the downstream valve, and where the LC system further comprises a downstream pump fluidically connected to the downstream valve and connectable to the at least one fluidic stream via the downstream valve in order to backflush and thereby clean the at least one HPLC column. A respective automated LC method is herein also disclosed.

LC system and method with column backflushing

The present disclosure is directed at an antibody conjugate having an antibody and a tag, wherein one or more element(s) present in the antibody exhibit an isotope ratio which differs from the naturally occurring isotope ratio of the one or more element(s), wherein the amount of the isotope which is less-common in nature, is increased to at least 4% of the atoms of the respective element in the antibody, as well as uses thereof.

Methods of protecting the sequence of an antibody conjugate from being determined

A method for detecting an analyte involving a) contacting the analyte, a capture agent and a solid surface to form a capture complex attached to a solid surface, b) cleaving at least one covalent bond within the analyte and/or within said capture agent in the capture complex and, thereby, releasing the analyte or a fragment thereof from the capture complex, and c) detecting at least one fragment of the analyte and, thereby, detecting the analyte is disclosed herein. A kit having i) a capture agent binding to an analyte; and ii) an agent cleaving at least one covalent bond within the analyte and/or within the capture agent; and to the use of a protease for releasing fragments of an analyte for detecting the analyte, wherein the analyte is bound to a solid surface via a capture agent is also disclosed herein.

Immobilized analytes

Copy protection for antibodies

The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.

Growing community of inventors

Oberhausen, Germany

Andreas Leinenbach