Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Amersham Biosciences Ab

Andreas Axén

Jean-Luc Maloisel

Nils Norrman

Gunnar Glad

Lars C Andersson

Anders Larsson

Enrique Napoleon Carredano

Henrik Ihre

Tesfai Gebru

Anna Grönberg

John Clachan

Herbert Baumann

Elles Steensma

Tania Ahmad

Helena Ohrvik

Martin Antti

The present invention relates to a computer implemented method () performed by a controller (C) configured to control a bioprocess comprised in a bioreactor (BR), the method comprising obtaining () measurement results by performing spectrophotometry of a bioprocessing fluid (FL), generating () control parameters based on the measurement results and one model and, controlling () the bioprocess using the generated parameters.

Method for control of a bioprocess

The present invention relates to an IgG-binding compound, which more specifically has affinity for human IgGs of &#x3ba;-type and functional derivatives thereof. More specifically, the compound according to the invention comprises an N,N-alkylated urea moiety located between an aromatic part and another part, which is a linear or cyclic substituted or unsubstituted aliphatic group. The compound binds to a pocket-shaped binding site present on all human IgG &#x3ba;-Fabs, which site is located between the two domains (CH1 and CL) of its constant part. Accordingly, the compound according to the invention is a ligand for human IgGs of &#x3ba;-type, and consequently, the invention also relates to a separation matrix for affinity chromatography, which matrix comprises said compound, as well as to other uses of the compound.

Use of urea variants as affinity ligands

The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R&#x2014;O&#x2014;CO&#x2014;(CH)&#x2014;CHNH&#x2014;CO&#x2014;OH wherein R is a selectively cleavable group; and n is an integer of 1&#x2013;4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

Preparation of a metal chelating separation medium

The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.

Method of generating metal chelating affinity ligands

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Uppsala, Sweden

Andreas Axén