Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Ge Healthcare Bioprocess R&d Ab

Cytiva Bioprocess R&d Ab

Andreas Axen

Nils Norrman

Anders Larsson

Gunnar Glad

Mathias Alterman

Henrik Ihre

Hanish Lakhani

Veena B N Rao

Victor Jose

John Clachan

Klaus Gebauer

Suma Memana Narayana Bhat

Jean-Luc Maloisel

Deepak Aravind

Bo-Lennart Johansson

Ronnie Palmgren

Lars C Andersson

Nagapriya Kavoori Sethumadhavan

Tobias Soderman

Enrique Napoleon Carredano

Jan Bergstrom

Jinyu Zou

Patric Fricking

Nicolas Thevenin

Dag Lindstrom

Anna Grönberg

Anders Hagvall

Ulrika Meyer

Camilla Estmer-Nilsson

Inger Andersson

Purbasha Halder

Tesfai Gebru

Linn Carlsson

Jonas Bengtsson

Lena Maria Sandberg

Peder Bergvall

Asa Rudstedt

Helena Lindgren

Anne Catharina Bergh

A bio-processing system () is presented. The bio-processing system () includes one or more bio-processing units (-), one or more process supporting devices (), one or more sensors (-), and a bio-processing workflow controller () wirelessly coupled to at least one of the one or more bio-processing units (-), the one or more process supporting devices (), and the one or more sensors (-). The bio-processing workflow controller () includes a processor () configured to create a reconfigurable bio-processing workflow, and where the reconfigurable bio-processing workflow is representative of an arrangement of one or more of the one or more bio-processing units (-), the one or more process supporting devices (), and the one or more sensors (-) to perform at least one bio-processing operation.

Method and system for creating reconfigurable bio-processing workflows

The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

Separation matrix and method of separation

A bio-processing system () for wirelessly powering one or more sensors (-) is presented. The system () includes bio-processing units (-), process supporting devices (-), energy sources (-), and sensors (-) including an energy harvesting unit () and an energy storage unit (). The system () includes a power management subsystem () wirelessly coupled to the sensors (-) and including a processor () configured to wirelessly monitor energy consumption of the sensors (-) and a level of energy stored in corresponding energy storage units (), select at least one sensor (-) based on the energy consumption of the sensors (-) and corresponding levels of energy stored in the energy storage units (), and identify at least one active energy source (-) as a power source, where the identified power source is configured to wirelessly transfer power to the selected sensor (-).

System and method for wirelessly powering a sensor in a bio-processing environment

The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Separation matrix

The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.

Cross-linked cellulose membranes

The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

Manufacture of chromatography matrices

The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands.

Method and device for small scale reactions

The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.

Process for cross-linking cellulose ester membranes

The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.

Separation matrix for viral purification

The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of

Method for generating metal chelating affinity ligands

The present invention relates to magnetic beads suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The magnetic beads are composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and these are then enclosed in a hydrophilic porous polymer, preferably agarose. This provides porous biocompatible beads without metal leakage. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer is preferably provided with ligands having affinity for selected biomolecules.

Magnetic beads comprising an outer coating of hydrophilic porous polymer and method of making thereof

The present invention relates to a chromatographic adsorbent for selectively adsorbing IgG, comprising the following formula and its corresponding enol-form, wherein X represents O, S, or NH; Rrepresents H, Calkyl, Calkoxy, Calkoxy-Calkyl, Ar, &#x2014;C(O)NHR, &#x2014;C(O)&#x2014;Ror halo; Rrepresents H, Calkyl or halo; Rrepresents H, Calkyl, Calkoxy, Calkoxy-Calkyl or Ar; n represents 0, 1, 2 or 3; Y represents a carrier. The present invention also relates to a method of producing said adsorbent as well as use thereof for separating substances by affinity chromatography.

[1, 2, 4] Triazolo [1, 5-A] pyrimidine derivatives as chromatographic adsorbent for the selective adsorption of IGG

The present invention relates to method of manufacturing a sulphonate-functionalized (S-functionalized) cation exchanger, which method comprises reacting olefinic groups with bisulphite in the presence of at least one amine oxide, such as N-methyl morpholine oxide (NMO). The olefinic groups of the carrier may be allyl groups; which may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

Manufacture of a chromatography matrix

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Uppsala, Sweden

Andreas Axen