Abbott Laboratories Corporation

Andreas A Kapsalis

Julian D Gordon

Emerson W Chan

Carl W Merkh

Kevin M Knigge

James D Defreese

John E Stephens

Werner Schulze

William G Robey

Brian P Braun

David S Vallaris

Benton A Durley, Deceased

Cynthia K Daluga

Joseph J Stojak, Ii

Richard E Thompson

Methods and kits are provided for the quantitative determination of the presence of an enzyme analyte in a sample by determination of the rate of analyte catalyzed reaction on a chromatographic medium. The methods may be used to determine both steady state and non-steady state enzyme reaction kinetics and provide a physical record reflecting the same. In a preferred embodiment, an analyte enzyme in a sample to be analyzed is immobilized at a reaction site on a chromatographic medium, the chromatographic medium is contacted with a solution containing a substrate, the solution is transported on the chromatographic medium to the reaction site where the analyte enzyme catalyzes the reaction of the substrate to produce a detectable reaction product at a rate related to the amount of enzyme present, and the solution and reaction product are transported from the reaction site to a detection region formed by a length of the chromatographic medium downstream from the reaction site. Transport continues until the solution reaches the end of the chromatographic medium or until the quantity of solution is exhausted.

Method of quantitative enzyme determination

An immunoassay which is capable of simultaneously detecting any desired number of antigens of one infectious agent, or combinations of antigens of several infectious agents, or any desired number of immunoglobulins of one infectious agent, or combinations of immunoglobulins of several infectious agents on a single solid support. A test sample is contacted with a solid support on which one or more antigens are immobilized as discrete test sites. Antigen-antibody complexes are formed and detected on the solid support.

Multilayer solid phase immunoassay support and method of use

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Evanston, IL, United States of America

Andreas A Kapsalis