Illumina Corporation

Illumina Cambridge Limited

Andrea Sabot

Harold Philip Swerdlow

Mark Edward Brennan Smith

Jean-Ernest Sohna Sohna

Adrian Martin Horgan

Isabelle Marie Julia Rasolonjatovo

Roberto Rigatti

Min-Jui Richard Shen

Mark T Reed

Isabelle Marie Julia Rasalonjatovo

The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

Method for selecting and amplifying polynucleotides

Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

Methods and compositions of localizing nucleic acids to arrays

The invention provides methods for generating a nucleic acid library. Target-specific primer-probes are hybridized to a target nucleic acid fragment to create a hybridization product. Each of the target-specific primer-probes comprises a target-specific sequence and a first adaptor sequence. The target nucleic acid fragment comprises a target genomic region of interest, wherein said target genomic region of interest comprises an exon of a gene and the target-specific primer-probes are tiled across the exon of the gene. The target nucleic acid fragment further comprises a second adaptor sequence different from said first adaptor sequence. Following hybridization, the target-specific primer-probes are extended to create double-stranded extension products and further amplified, thereby generating the nucleic acid library.

Methods for selecting and amplifying polynucleotides

Methods of localizing nucleic acids to arrays

The present invention comprises systems and devices for isothermal amplification of polynucleotide sequences to produce DNA cluster arrays.

Devices and systems for creation of DNA cluster arrays

Methods are disclosed relating to localizing nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

A composition including (a) a solid support having a surface; (b) a first plurality of nucleic acids immobilized on the surface, wherein the nucleic acids in the first plurality each include the P7 primer sequence (5'-CAAGCAGAAGACGGCATACGA-3&#x2032;; SEQ ID NO: 3); and (c) a second plurality of nucleic acids immobilized on the surface, wherein the nucleic acids in the second plurality each include the P5 primer sequence (5&#x2032;-AATGATACGGCGACCACCGA-3&#x2032;; SEQ ID NO: 4).

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Essex, United Kingdom

Andrea Sabot