Basf Se Corporation

Evonik Degussa Gmbh

Wintershall Holding Gmbh

Basf Aktiengesellschaft

Bhs Corrugated Maschinen-und Anlagenbau Gmbh

Andrea Herold

Oskar Zelder

Hartwig Schröder

Stefan Haefner

Corinna Klopprogge

R Rogers Yocum

Mark K Williams

Christian Fleck

Thomas A Patterson

Hartwig Schroder

Mari Granström

Weol Kyu Jeong

Julia Kristiane Schmidt

Beata Brockmann

Karl-Heinz Schumacher

Wolfgang Grabarse

Janice G Pero

Gledison Fonseca

Wolfgang Gaschler

Stephan Freyer

Oliver Hartz

Christoph Wittmann

Rabie Al-Hellani

Thomas Letzelter

Jörg Therre

Uwe Sauer

Tobias Käppler

Elmar Heinzle

Hubertus Kröner

Sascha Rollie

Florian Lehr

Bernd Leonhardt

Andrea Hlubek

Carsten Schwalb

Theron Herman

Jens Kroemer

Anja Knietsch

Jörg Mampel

Hansjörg Rettenmaier

Hildegard Stein

Ellen Krüger

Hartwig SCHRÖDER

Mari GRANSTRÖM

Mark Williams

The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of &#x2265;2.0 to &#x2264;4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.

Method for preparing an aqueous solution of beta-glucan

The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-&#x3b2;-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-&#x3b2;-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-&#x3b2;-D-glucan synthase-activity or the use of such a polypeptide for producing &#x3b2;-glucans. Furthermore, the present invention relates to methods for producing &#x3b2;-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-&#x3b2;-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-&#x3b2;-D-glucan synthase-activity into a microorganism being able to synthesize &#x3b2;-glucans.

Genetically modified microorganisms capable of producing &#x3b2;-glucans and methods for producing &#x3b2;-glucans

Genetically modified microorganisms capable of producing beta-glucans and methods for producing beta-glucans

Described is the use of synthetic adhesives in the manufacture of corrugated fiberboard at relatively low temperatures and high lineal speeds. The corrugated fiberboard includes a corrugated sheet of paper and a flat linerboard and the corrugation of the corrugated sheet of paper is produced at paper temperatures below 95&#xb0; C. and at a lineal speed above 150 m/min. The production of the corrugation of a corrugated sheet of paper is immediately followed by a continuous operation in which a preferably unheated corrugated board adhesive is applied and the corrugated sheet of paper is adhered to at least one first linerboard. The corrugated board adhesive used is an aqueous adhesive dispersion based on at least one synthetic, dispersed polymer having preferably more than 40% by weight solids content, selected from acrylate copolymers, copolymers of vinylaromatics and conjugated aliphatic dienes and vinyl acetate-alkylene copolymers, wherein the glass transition temperatures of the polymers are above 20&#xb0; C. and (preferably at least 5&#xb0; C.) below the surface temperature of the corrugated sheet of paper to which they are applied.

Use of synthetic adhesives in the manufacture of corrugated fiberboard

The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.

Process for the production of gamma-aminobutyric acid

Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

Process for the production of cadaverine

The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &#x394;metF organism or a &#x394;metE AmetH organism, for example, mutants ofor, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &#x394;metF &#x394;metB strain ofcan use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

Use of dimethyl disulfide for methionine production in microoraganisms

The present invention relates to nucleotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof that are modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

Nucleic acid encoding a cobalamin-dependent methionine synthase polypeptide

The present invention relates to microorganisms and methods for producing methionine by reactivation of the MetH enzyme.

Microorganisms with a reactivation system for cob(I)alamin-dependent methionine synthase

The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Microorganism and process for the preparation of L-methionine

The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

Multiple promoters and the use thereof for gene expression

Growing community of inventors

Ketsch, Germany

Andrea Herold