Lumicks Ca Holding B.v.

Stichting Vu

Stichting Vu-vumc

Lumicks Dsm Holding B.v.

Andrea Candelli

Gerrit Sitters

Felix Oswald

Gijs Jan Lodewijk Wuite

Erwin Johannes Gerard Peterman

Iddo Heller

Andreas Sebastian Biebricher

Niels Laurens

Stefan Walter Hell

Mattijs De Groot

A method of manipulating and/or investigating cellular bodies () is provided. The method comprises the steps of: providing a sample holder () comprising a holding space () for holding a fluid medium (); providing a sample () comprising one or more cellular bodies () in a fluid medium () in the holding space (); generating an acoustic wave in the holding space exerting a force (F) on the sample () in the holding space (). The method further comprises providing the holding space () with a functionalised wall surface portion () to be contacted by the sample () and the sample () is in contact with the functionalised wall surface portion () during at least part of the step of application of the acoustic wave. A system and a sample holder () are also provided.

Method and system for studying biological cells

The present disclosure relates to an apparatus () and method for controlling a plurality of simultaneously active optical traps (OT,OT,OT). In one method, trapping beams (TB,TB,TB) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT,OT,OT) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L,L) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB), wherein the detector beam has a distinct wavelength (&#x3bb;A) from that of the overlapping trapping beam (TB). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (&#x3bb;A) of the detector beam (AB) is measured.

Apparatus and method for controlling a plurality of optical traps

The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X) of the excitation focus (EF) along the scanning line (SL).

Method and system for imaging a molecular strand

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Amsterdam, Netherlands

Andrea Candelli