The University of British Columbia

Precision Nanosystems Inc.

Precision Nanosystems Ulc

Andre Wild

Timothy Leaver

Robert James Taylor

Pieter Rutter Cullis

Nathan Maurice Belliveau

Shao Fang Shannon Chang

Euan Ramsay

Igor V Jigaltsev

Colin Walsh

Kevin Ou

Shao Fang Chang

Pieter R Cullis

Carl Lars Genghis Hansen

Benjamin Albert Versteeg

Jens Huft

David E Walker

Keara Marshall

Stuart Malcolm

Ismail Hafez

James R Taylor

Alex Leung

Rodney Paul Bucknell

Aysha Ansari

Ke Ma

Sukhjinder Singh Sidhu

Nathan M Belliveau

Anitha Thomas

Phillip Higgins

Nicolas Klaassen

Gesine Heuck

Niklaus Alexander Andre

Andrew Robert Patterson

Thomas William Cameron Debenham

R James Taylor

Euan Ramsey

David Walker

Stuart Malcom

James Taylor

Limit size lipid nanoparticles, methods for using the lipid nanoparticles, and methods and systems for making limit size lipid nanoparticles.

Limit size lipid nanoparticles and related methods

The present disclosure is directed towards improved systems and methods for large-scale production of nanoparticles used for delivery of therapeutic material. The apparatus can be used to manufacture a wide array of nanoparticles containing therapeutic material including, but not limited to, lipid nanoparticles and polymer nanoparticles. In certain embodiments, continuous flow operation and parallelization of microfluidic mixers contribute to increased nanoparticle production volume.

Continuous flow microfluidic system

A 'smart' instrument for mixing (), a microfluidic chip (), and a system wherein they are used to prepare formulations is provided. The microfluidic chip comprises microchannels and a programmable data component. The system achieves optimal formulations for RNA, antisense, peptides and small molecules in the hands of even novice users.

Smart microfluidic mixing instrument and cartridges

Microfluidic mixing devices and systems

Disclosed herein are fluidic mixers having bifurcated fluidic flow through toroidal mixing elements. The mixers operate, at least partially, by Dean vortexing. Accordingly, the mixers are referred to as Dean Vortex Bifurcating Mixers ('DVBM'). The DVBM utilize Dean vortexing and asymmetric bifurcation of the fluidic channels that form the mixers to achieve the goal of optimized microfluidic mixing. The disclosed DVBM mixers can be incorporated into any fluidic (e.g., microfluidic) device known to those of skill in the art where mixing two or more fluids is desired. The disclosed mixers can be combined with any fluidic elements known to those of skill in the art, including syringes, pumps, inlets, outlets, non-DVBM mixers, heaters, assays, detectors, and the like.

Bifurcating mixers and methods of their use and manufacture

The present disclosure is directed towards a disposable microfluidic cartridge configured for use in a system for the small scale production of nanoparticles used in scientific research or therapeutic applications. The system can be used to produce a wide variety of nanoparticles, including but not limited to lipid and polymer nanoparticles, carrying a variety of payloads. The system provides for a simple workflow which in certain embodiments can be used to produce a sterile product.

Disposable microfluidic cartridge

Microfluidic chip

Transfection reagent composition, lipid nanoparticles prepared from the transfection reagent composition, kits that include the transfection reagent composition, and methods for making and using lipid nanoparticles prepared from the transfection reagent composition. Lipids when dispersed in aqueous media readily form liposomes, such as unilamellar vesicles and multilamellar vesicles. Liposomes have been used successfully to encapsulate and deliver a wide range of chemicals including nucleic acids, proteins and small molecule drugs, to cells.

Lipid nanoparticles for transfection and related methods

Methods and devices for making particles at small volumes.

Device for formulating particles at small volumes

Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.

Nucleic acid-containing lipid particles and related methods

Growing community of inventors

Vancouver, Canada

Andre Wild