Akademie Der Wissenschaften Der Ddr

Andre Rosenthal

Hans-Dieter Hunger

Horst Kagelmaker

Monika Gratschus

Monika Graatschus

A device for solid phase sequencing of nucleic acid segments comprises a plurality of sequencing blocks for storaging and processing n nucleic acid fragments immobilized on carrier segments or in homogenous phase during a sequencing process, each of a sample dosing device for immobilizing n labeled nucleic acid samples from the sequencing blocks onto areas of a carrier matrix in a simultaneous fashion and including a capillary holder and a plurality of capillaries corresponding to the number n of different nucleic acid samples to be sequences and arranged according to the distribution of the reaction vessels with a distance between capillary orifices from the capillary holder greater than a depth of the reaction vessels, a plurality of carrier holders for fixing carrier matrices in a predetermined position during the immobilization of the nucleic acid samples with the sample dosing device and each having a recess corresponding to respective dimensions of the carrier matrix and a plurality of bores with a number and distribution pattern corresponding to those of the reaction vessels and with a diameter which is somewhat larger than a diameter of the capillary, a punch for simultaneous punching of n immobilized and chemically modified nucleic acid samples as individual carrier segments out of the carrier matrix into the sequencing blocks and including a punching plate and a plurality of punching pins with a number and distribution pattern which also corresponds to those of the reaction vessels.

Device for solid phase sequencing of nucleic acid fragments

A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.

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Berlin, Germany

Andre Rosenthal