Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Andre Lieber

Michael Strauss

Michael Strauss, Deceased

The invention is an expression cassette for the antisense expression of ribozyme, having a strong promotor, suitably a T7 promotor, an adenoviral va-RNA gene, a stable loop region, and an insertion site for the antisense/ribozyme sequence in the loop region.

Expression cassette for antisense expression of ribozyme

A ribozyme library which comprises a collection of ribozyme genes encoding a hammerhead structure and flanking sequences of random nucleotides cloned at least once into an expression cassette for ribozyme expression.

Ribozyme gene library and method for making

Agents for stimulating the dividing activity of mammalian cells are presented, which have as an active substance a nucleotide sequence with a complimentary sequence (anti-sense) to a domain of the mRNA of tumor suppressor retinoblastoma or p16 genes acting on cell dividing activity. For Rb, the agents are nucleotide sequences 5'-GGTTTTGGGCGGCATGAC-3' (SEQ ID NO: 1) and 5'-CTCAGTAAAAGTGAACGA-3' (SEQ ID NO: 2); a nucleotide sequence comprising 49 base pairs, wherein the domain of the mRNA comprises nucleotides 124 to 152; and a 360 base pair nucleotide wherein the domain of the mRNA comprises an Hpa I restriction endonuclease site, an ASP718 endonuclease site, a portion of the 5' untranslated domain of the Rb gene, and a first exon of the Rb gene. For p16, the agent comprises GATCCATGCT GCTCC (SEQ ID NO: 3).

Growing community of inventors

Berlin, Germany

Andre Lieber