Johns Hopkins University

Ucsd Tech Transfer Office

The United States of America, as Represented by the Secretary, Department of Health and Human Services

Andre Levchenko

Andrew P Feinberg

Dan L Longo

Alexander Groisman

Steven An

Onur Kilic

Raymond Cheong

Chiaochun J Wang

Saurabh Paliwal

Minoru Sh Ko

The present invention provides an integrated microphysiological device and fabrication methods thereof, as well as methods of use to perform biological assays.

Device and method for analysis of tissue constructs

Cell stimulation, staining, and visualization are common techniques in both clinical and research settings. The invention is directed to microfluidic devices for in situ cell stimulation, staining, and/or visualization, and related methods for applying one or more stimuli to the cells, and fixing and staining of cells in situ. The device allows for high-throughput screening of living cells using a minimal quantity of reagents where the fate of individual cells can be followed over time.

Device for high-throughput stimulation, immunostaining, and visualization of single cells

The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.

Microfluidic device for high-throughput cellular gradient and dose response studies

Growing community of inventors

Ellicott City, MD, United States of America

Andre Levchenko