Solvay Enzymes Gmbh & Co. Kg

Kali-chemie Aktiengesellschaft

Gesellschaft Fuer Biotechnologische Forschung Mbh

Gesellschaft Fuer Bio-technologische Forschung

Andre Clippe

Antoine Amory

Detlef Wilke

Roman Vetter

Gerhard Konieczny-Janda

Wolfgang Aehle

Dietmar Schomburg

Ingo Muecke

Amory Antoine

Harald Sobek

The use of alkaline bacillus proteases in commercial laundry methods and compositions containing these proteases for commercial laundering are described.

Use of alkaline proteases in industrial textile laundering processes

Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.

Stabilized enzymes and process for preparing them

Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

Highly alkaline proteases

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Brussels, Belgium

Andre Clippe