Institut Pasteur

Other

Université Pierre Et Marie Curie

Cellectis

Cellectis S.a.

Centre National De La Recherche Scientifique

Children's Medical Center Corporation

Institut Curie

Universite Paris-Vi

Institut Pasteur Universite Paris-Vi

Universite Peirre Et Marie Curie

Andre Choulika

Bernard Dujon

Arnaud Perrin

Jean-Francois Nicolas

Cecile Fairhead

Anne Plessis

Laurence Colleaux

Agnes Thierry

Jean-Pierre Cabaniols

Jean-Charles Epinat

Sylvie Robine

Daniel Louvard

Emmanuel Lacroix

Patrick Chames

Sylvain Arnould

Charles Babinet

Christophe Delenda

Michel Cohen-Tannoudji

Frederic Jaisser

Philippe Duchateau

Laurent Poirot

Richard C Mulligan

Julianne Smith

Agnes Gouble

David Sourdive

Frederic Paques

Christophe Perez-Michaut

Sylvia Bruneau

Jean-Stephane Joly

Violette Thermes

Filomena Ristoratore

Methods of modifying, repairing, attenuating and inactivating a gene or other chromosomal DNA in a cell are disclosed. Also disclosed are methods of treating or prophylaxis of genetic disease in an individual in need thereof. Further disclosed are chimeric restriction endonucleases.

Chromosomal modification involving the induction of double-stranded DNA cleavage and homologous recombination at the cleavage site

The invention relates to a set of genetic constructs which allow the efficient and reproducible introduction of a specific nucleotide sequence at a fixed position in the genome by generating a double strand break at a specific position in the genome using a meganuclease and so stimulating a homologous recombination event at this locus between the genomic site and a transfected donor sequence. The present invention also relates to methods using these constructs and to these materials in the form of a kit.

Meganuclease recombination system

This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare-cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare-cutting endonuclease for genetic engineering and gene therapy.

Hybrid and single chain meganucleases and use thereof

A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y (SEQ ID NO: 23 is residues 1-153 of pdb Ig9y) and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y (SEQ ID NO: 23 is residues 1-153 of pdb Ig9y) in a single polypeptide.

Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof

An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.

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Nucleotide sequence encoding the enzyme I-Scel and the uses thereof

Nucleotide sequence encoding the enzyme I-SceI and the uses thereof

The present invention concerns a method for in vivo generation of a linear polynucleotide with 5' and 3&#x2032; free ends from a vector having no free end, said linear polynucleotide being integrated into the host cell genome. The vector having no free end according to the present invention comprise the polynucleotide to be linearized or excised flanked by a cleavage site, said cleavage site being preferably not found in the host cell genome. The present invention also relates to the resulting cells and their uses, for example for production of proteins or other genes, biomolecules, biomaterials, transgenic plants, vaccines, transgenic animals or for treatment or prophylaxis of a condition or disorder in an individual.

Random integration of a polynucleotide by in vivo linearization

Nucleotide sequence encoding the enzyme I-SecI and the uses thereof

Nucleotide sequence encoding the enzyme I-SCEI and the uses thereof

The invention relates to methods of introducing a heterologous DNA sequence into a mouse embryonic stem cell wherein the DNA sequence is inserted by homologous recombination into a villin gene/I-SceI hybrid by creating a double strand break with I-SceI meganuclease. Subsequently, the mouse embryonic stem cells can be used to generate a transgenic mouse comprising the heterologous DNA sequence. Additionally, the methods can be used for gene replacement in ovo where a mouse oocyte containing a villin gene/I-SceI hybrid within its genome exists or is first generated. More generally, the methods can be used for the targeted insertion of a heterologous DNA sequence into any cell containing a villin gene/I-SceI hybrid sequence within its genome.

I-Sce I induced gene replacement and gene conversion in embryonic stem cells

Nucleotide sequence encoding the enzyme I SceI and the use thereof

Growing community of inventors

Paris, France

Andre Choulika