Dana-farber-cancer Institute Inc.

Cell Genesys, Inc.

Gbp Ip, LLC

Anatoly Bukovsky

Deborah A Farson

Thomas L Dull

Luigi Naldini

Rochelle Witt

Heide Reil

Heinrich G&ouml;ttlinger

Lentiviral vectors modified at the 5' LTR or both the 5&#x2032; and 3&#x2032; LTR are useful in the production of recombinant lentivirus vectors (See the Figure). Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production. The vectors can contain inducible or conditional promoters.

Method and means for producing high titer, safe, recombinant lentivirus vectors

Vector systems that encode viral proteins that will assemble into an infectious lentivirus particle having a deletion of the lentiviral matrix are described. In one embodiment the vectors encode a lentiviral envelope protein that contains a deletion of the cytoplasmic tail. In a second embodiment a heterologous envelope as compared to the lentivirus is used such as an envelope protein that targets an endocytic compartment.

Infectious pseudotyped lentiviral vectors lacking matrix protein and uses thereof

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Bellevue, WA, United States of America

Anatoly Bukovsky