Drexel University

University of Pennsylvania

The Children's Hospital of Philadelphia

Anat Perets

Peter I Lelkes

Mengyan Li

Philip Lazarovici

Honesto Poblete

Robert J Levy

Russell Composto

Pimporn Uttayarat

Methods of making a biologically active three-dimensional scaffold capable of supporting growth and differentiation of a cell are described. Biologically active three-dimensional scaffold made by the methods of the invention and an engineered tissue made from the scaffolds are described. Fibers of desired porosity can be obtained from non-structural ECM by lyophilization and/or electrospinning which can be useful for numerous tissue engineering applications requiring complex scaffolds, such as wound healing, artificial skin (burns), soft tissue replacement/repair and spinal cord injury.

Three-dimensional scaffolds for tissue engineering made by processing complex extracts of natural extracellular matrices

The present invention relates to a hybrid graft and methods of generating the hybrid graft. The hybrid graft comprises an exterior surface and a luminal surface. The luminal surface comprises a micropattern of grooves to which cells adhere and orient along. The exterior surface comprises electrospun microfibers wherein the microfibers provide mechanical properties to the graft. The hybrid graft is capable supporting endothelial cell attachment, endothelial cell alignment, cell proliferation, and maintaining their in vivo function. The graft of the invention can recapitulate the in vivo morphology and function of natural vascular endothelium.

Small diameter vascular graft produced by a hybrid method

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Narberth, PA, United States of America

Anat Perets