Melt & Marble Ab

Biopetrolia Ab

Chrysea Limited

Total Raffinage Chimie

Anastasia Krivoruchko

Jens Nielsen

Florian David

Tao Yu

Verena Siewers

Yiming Zhang

Zongjie Dai

Mingtao Huang

Bo Jiang

Yong-Jin Zhou

Jiufu Qin

Leonie Wenning

Zhiwei Zhu

The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

Fungal cell with improved protein production capacity

The present invention generally related to a modified microbial cell capable of producing high levels of spermidine and/spermidine derivatives. The genetically modified microbial cell comprises at least one modification to native spermidine biosynthetic pathway via putrescine together with genes involved in the S-adenosylmethionine biosyntheticpathway.

Microbial cells for spermidine production

A fungal cell capable of producing high levels of fatty acids and fatty acid-derived products comprises at least one modification to the endogenous fatty acid metabolism.

Yeast cells genetically modified for downregulation of pyruvate decarboxylase activity and FBP-sensitive pyruvate kinase

A fungal cell is capable of producing high levels of fatty acids and fatty acid-derived products. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

Genetically modified fungal cells for overexpression of an acetyl-CoA carboxylase and a pyruvate carboxylase

The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

Engineering of acetyl-CoA metabolism in yeast

The present invention relates to a method for producing fatty acids, in particular shot/medium-chain fatty acids (S/MCFA), by expression in a host cell of a recombinant fatty acid synthase (FAS) naturally devoid of a thioesterase (TE) domain comprising a heterologous thioesterase (TE). The invention further relates to polynucleotides comprising such recombinant FAS, polypeptides encoded thereby, and vectors comprising such polynucleotides, as well as recombinant host cells comprising the polynucleotides, polypeptides, or vectors.

Method of producing fatty acids

The present invention generally relates to a genetically modified fungal cell capable of producing a very long, chain fatty acid (VLCFA) and/or a VLCFA derivative. The genetically modified fungal cell comprises at least one exogenous gene encoding a fatty acyl-CoA reductase, and at least one gene encoding an elongase, and/or at least one gene encoding a fatty acid synthase.

Fungal cells and methods for production of very long chain fatty acid derived products

The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine 'acetylated derivatives cycle', L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

Growing community of inventors

Gothenburg, Sweden

Anastasia Krivoruchko