Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Horiba Abx Sas

Horiba Abx Sas / Horiba Ltd

Anais Ali-Cherif

Pierre Blandin

Aurelien Daynes

Estelle Gremion

Yves Fouillet

Olivier Cioni

Damien Isebe

Sebastien Raimbault

Sébastien Raimbault

Aurélien Daynes

A device for preparing a solution from a sample and a reagent, the device includes a microfluidic array having a sample supply inlet, a reagent supply inlet, a discharge outlet, a solution collection outlet, a sampling zone to which the inlets are connected, first and second preparation chambers connected to the sampling zone, arranged to either side of the sampling zone such that the liquid flowing from one preparation chamber to the other flows through the first sampling zone, the first preparation chamber having a volume that is variable between a minimum volume and a calibrated volume. The device includes valves interrupting the flow of the fluid at least at the two inlets and the collection and discharge outlets.

Microfluidic sample preparation device offering high repeatability

The invention is a method for estimating a representative volume of particles of interest () immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (), the light source emitting an incident light wave () propagating towards the sample () along a propagation axis (Z); b) acquiring, by means of an image sensor (), an image (I) of the sample (), formed in a detection plane (P), the sample being arranged between the light source () and the image sensor (), each image being representative of a light wave () referred to as an exposure light wave, to which the image sensor () is exposed under the effect of illumination; c) using the image of the sample (I), acquired during step b), and a holographic propagation operator, to calculate a complex expression (A (x, y, z)) of the exposure light wave () in different positions relative to the detection plane; the method comprising a step of estimating the representative volume (AA) of the particles of interest () depending on the complex expressions calculated during step c).

Optical method for estimating a representative volume of particles present in a sample based on a size of each region of interest

The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Method for counting particles in a sample by means of lensless imaging

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Clermont-Ferrand, France

Anais Ali-Cherif