Centro De Ingenieria Genetica Y Biotecnologia

Centro De Ingenieria Genetica Y Biotechnologia

Anabel Alvarez Acosta

Consuelo Nazabal Galvez

Diogenes Quintana Vazquez

Alexis Musacchio Lasa

Vivian Morera Cordova

Alejandro Miguel Martin Dunn

Maria De Jesus Leal Angulo

Maria De Angulo

Recardo De La Caridid Siva Rodriquez

Selman H Sosa

Saturnino H Martinez

Enrique Gerardo Guillen Nieto

Guillen Nieto

Juan M Grillo

Recardo De La Caridid Siva Rodriguez

Carmen Elena Gomez Rodriquez

Alejandro Miguel Dunn

Carlos Antonio Durate Cano

Silva Rodriquez

Beatriz T Santos

Lidia I Perez

Enrique Gerardo Nieto

Luis Emilio Carpio Munoz

Sonia G Blanco

Carlos Antonio Cano

Jesus A Del Valle Rosales

Evelin C Menendez

Luis Emilio Munoz

Julio R Maso

Edelgis C Rodriquez

Carmen Elena Rodriquez

Silian C Leon

The present invention related to biotechnology and genetic engineering, particularly the expression of proteins of viral origin in microorganisms through their fusion by applying recombinant DNA technology to bacterial peptides. The present invention provides an effcient process for the expression inof heterlogous proteins as fusion peptides with a view to obtaining them with a high degree of purity, in commercially useful amounts, and in an appropriate form for their inclusion in vaccine preparations. What is essentially used is a stabilizing sequence derived from the first 47 amino acids of the antigen P64k ofB:4:P1.15. In particular, use is made of a recombinant plasmid containing said sequence, under the control of the tryptophane promoter ofand of the terminator of the transcription of the phage T4, including restrictions sites which provide for the cloning in phase of DNA fragments coding for polypeptides of interest.

Monoclonal antibody to the stabilizer peptide of the P64K antigen of Neisseria meningitidis

System for the expression of heterologous antigens as fusion proteins

The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64,000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.

Nucleotide sequence coding for an outer membrane protein from Neisseria

Growing community of inventors

Habana, Cuba

Anabel Alvarez Acosta