Lockheed Martin Corporation

University of Virginia Patent Foundation

Leidos Innovations Technology, Inc.

Microlab Diagnostics, Inc.

An-Chi Tsuei

James P Landers

Brian Root

Matthew Daniel Solomon

Orion N Scott

Joan Marie Bienvenue

David Saul

Jason Paul Hayes

Peter Van Ruijven

Douglas J South

Michael E Egan

Matthew Springer

Paul Kinnon

Daniel G Mills

Jingyi Li

Christopher Birch

Jacquelyn A DuVall

Brandon L Thompson

Daniel A Nelson

Delphine Le Roux

Michael Egan

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

Devices and methods for extraction, separation and thermocycling

A microfluidic cartridge can include at least one nucleic acid analysis portion. Each nucleic acid analysis portion can include a fluidic network being configured for micro-liter volumes or less, a sample input at the beginning of the fluidic network, a plurality of vent ports and fluidic channels in the fluidic network configured to effectuate hydrodynamic movement within the fluidic network, an extraction mixture reservoir in the fluidic network, a mixing chamber in the fluidic network, an amplification chamber in the fluidic network, and a separation channel in the fluidic network. A nucleic acid analyzer can be capable of performing nucleic acid analysis using the microfluidic cartridge. A nucleic acid analysis method can be performed using the microfluidic cartridge.

Microfluidic cartridge

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An-Chi Tsuei