Weyerhaeuser Nr Company

Amy M Jamruszka-Lewis

Robert A Starr

Anthony P Swanda

Methods of transferring a plurality of plant somatic embryos to germination medium are provided.

Methods of transferring plant embryos to germination medium

The present disclosure provides methods of separating plant germinants from gelled germination medium.

Method of separating plant germinants from gelled media

The present invention is directed to an automated system and methods for separating and singulating plant embryos.

Automated system and methods for separating and singulating plant embryos

In one aspect, the present invention provides methods of producing a synchronized population of conifer somatic embryo germinants in preparation for transplantation. The methods of the invention comprise the steps of: (a) culturing conifer somatic embryos on sterile germination medium for a sufficient period of time to produce germinants comprising a visible, well-defined epicotyl stem of a desired length; (b) selecting a plurality of germinants having an epicotyl stem of the desired length; (c) storing the selected germinants having an epicotyl stem of the desired length produced in step (a) while still on the sterile germination medium from step (a) in a cold environment in which the temperature is in the range of 0.5&#xb0; C. to 10&#xb0; C. for a time period up to six months; and (d) transferring the germinants stored according to step (c) from the germination medium into water for a time of up to about four weeks at a temperature of about 0.5&#xb0; C. to about 5&#xb0; C. prior to planting the selected germinants into growth medium.

Methods of producing a synchronized population of conifer somatic embryo germinants

In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5&#xb0; C. to 10&#xb0; C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24&#xb0; C. prior to transplantation into growth medium.

Growing community of inventors

Sumner, WA, United States of America

Amy M Jamruszka-Lewis