E.i. Dupont De Nemours and Company

Genencor International, Inc.

Amy K Hsu

Mark Scott Payne

Fernando Valle

Philippe Soucaille

Stephen K Picataggio

Donald Eugene Trimbur

Ramesh V Nair

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Promoter and plasmid system for genetic engineering

Growing community of inventors

Redwood, CA, United States of America

Amy K Hsu