Monsanto Technology LLC

Monsanto Company

Ecogen, Inc.

Amy Jelen Gilmer

Thomas M Malvar

James Arthur Baum

Anne-Marie Light Mettus

William P Donovan

Mark J Rupar

Chih-Rei Chu

Ann-Marie Light Mettus

Disclosed are novel synthetically-modifiedchimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

Broad-spectrum &#x3b4;-endotoxins and method and kit for detecting same and for detecting polynucleotides encoding same

Disclosed arestrains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novelgenes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

[object Object]

Method of detecting broad-spectrum delta-endotoxins

Disclosed are novel synthetically-modifiednucleic acid segments encoding &#x3b4;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &#x3b1; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

Methods for generating lepidopteran-toxic polypeptides

Disclosed are novel synthetically modifiedchimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

Hybrid Bacillus thuringiensis &#x3b4;-endotoxins with novel broad-spectrum insecticidal activity

Antibodies immunologically reactive with broad-spectrum delta endotoxins

Disclosed are novel synthetically-modified nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

Nucleic acid and polypeptide compositions encoding lepidopteran-toxic polypeptides

Compositions encoding lepidopteran-toxic polypeptides and methods of use

Disclosed are novel synthetically-modified chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

Antibodies immunologically reactive with broad-spectrum &dgr; endotoxins

Disclosed, are novel synthetically-modified chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

Polynucleotide compositions encoding broad-spectrum &dgr; endotoxins

Disclosed are strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Growing community of inventors

Langhorne, PA, United States of America

Amy Jelen Gilmer