University of Colorado

The University of Tennessee Research Foundation

National Institute of Standards and Technology

Institute of Organic Chemistry Pos

Amy E Palmer

Ralph Jimenez

Robert T Batey

Lloyd M Davis

Dorota Gryko

Kevin Dean

Esther Braselmann

Jennifer Lubbeck

This invention relates to the field of ribonucleic acid (RNA) regulation of intracellular activity. In particular, the invention relates to compositions and methods of identifying and tracking specific intracellular RNAs. For example, a fluorescently tagged RNA probe may be tracked by in vivo live imaging throughout its intracellular lifetime in order to determine its purpose and identify regulatory targets to modify its effects. Alternatively, an RNA probe may carry a therapeutic payload for treatment of medical condition or disorder.

Compositions and methods for tagging ribonucleic acids

Devices and methods for screening emissive properties of a cell, such as the resistance to photobleaching or other photophysical property. In one example, a device may include a microfluidic reservoir having at least an input channel for receiving the cell, a main channel fluidly coupled with the input channel, at least a first output channel and a second output channel, the first and second output channels fluidly coupled with the main channel; and a multibeam interrogation section generating a plurality of light beams impinging upon the main channel of the microfluidic reservoir. As a cell passes from the input channel through the main channel of the microfluidic reservoir, the cell is exposed to the plurality of light beams thereby generating emissions that are received by a signal processing section. A cell trapping section selectively diverts the cell to the second output channel if the cell contains desired emissive properties.

Optically integrated microfluidic cytometers for high throughput screening of photophysical properties of cells or particles

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Boulder, CO, United States of America

Amy E Palmer