Technion Research & Development Foundation Ltd

Tel Aviv University

Amit Meller

Tal Gilboa

Joshua Spitzberg

Moran Bercovici

Xander Frank Van Kooten

Yuval Ebenstein

Elmar Weinhold

Assaf Grunwald

Ossama Assad

Michelle Patkin

Chen Torfstein

Yael Michaeli-Hoch

Arik Girsault

Yana Ben Zvi Rozevsky

Adam Zrehen

Eran Zvuloni

Yana Rozevsky

The present invention concerns compositions, kits and methods for genetic variation detection and classification. These methods combine the specificity of a ligation reaction with the single-molecule sensitivity of nanopore biosensors. This invention can achieve clinical detection of many diseases, including bacterial infections and cancer entities, by identification of specific genetic alterations.

Compositions and methods for detection of genomic variations

Systems comprising a light source, thin membrane immersed in an aqueous solution and a system to direct and focus light from the light source to a spot on the membrane are provided. Methods of thinning and etching a membrane are also provided, as are membranes comprising a nanopore with a Gaussian curve shaped cross-section.

Nanopore fabrication

Systems for detecting fluorescence from a molecule comprising an ion-impermeable film comprising at least one ion-conducting nanopore; a first and second liquid reservoir separated by the film; a means to induce movement of the molecule from the first reservoir to the second reservoir via the nanopore; a light source capable of exciting the molecule to emit fluorescence, wherein the light source shines into the second reservoir; a metallic layer adhered to the film by an adhesion layer and comprising a nanowell structure located adjacent to the nanopore; and a detector configured to detect the fluorescence emitted by the molecule are provided. Methods of use of the systems are also provided.

Light-enhancing plasmonic nanowell-nanopore biosensor and use thereof

Methods and kits for detecting the presence of at least one target DNA sequence with or without a modification in a DNA molecule are provided.

Methods of detecting modified and unmodified DNA

Devices for detecting a molecule of interest comprising an electrokinetic focusing apparatus and a nanopore apparatus are provided. Kits and systems comprising the apparatus are also provided; as are methods of detecting molecules of interest comprising running the molecules through the electrokinetic focusing apparatus and then detecting the focused molecules as they pass through the nanopore.

Devices and methods for improved single-molecule detection

Growing community of inventors

Haifa, Israel

Amit Meller