S.d. Sight Diagnostics Ltd.

Amir Zait

Joseph Joel Pollak

Yochay Shlomo Eshel

Sharon Pecker

Dan Gluck

Sarah Levy Schreier

Arnon Houri Yafin

Sarah Levy

Trevor Ruggiero

Daniel Levner

Natalie Lezmy

Noam Yorav-raphael

Yonatan Halperin

Peretz Yafin

Itamar Weiss

Apparatus and methods are provided including imaging a blood sample that is a cell suspension deposited in a sample chamber. The cells are allowed to settle in the sample chamber to form a monolayer of cells. At least one microscopic image is acquired of the monolayer of cells using a microscope () while the microscope is focused at a monolayer-depth-level, and a first platelet count of platelets that have settled within the monolayer, is determined. An additional microscopic image of the simple is acquired, while the microscope is focused at a different depth level from the monolayer-depth-level, and a second platelet count of platelets that have not settled within the monolayer is determined. An output is generated based upon the first and second platelet counts. Other applications are also described.

Detecting platelets in a blood sample

Apparatus and methods are described including preparing a blood sample for analysis by depositing the blood sample within a sample chamber (), and placing the sample chamber, with the blood sample deposited therein, within a microscopy unit (). One or more microscopic images of the sample chamber () with the blood sample deposited therein are acquired, using a microscope of the microscopy unit. Based upon the one or more images, an amount of one or more cell types within the sample chamber that had already settled within the sample chamber, prior to acquisition of the one or more microscopic images is determined. A characteristic of the sample is determined, at least partially in response thereto. Other applications are also described.

Accounting for errors in optical measurements

Apparatus and methods are described including measuring hematocrit within a blood sample, by performing a first measurement on a first portion of the blood sample. Mean corpuscular volume in the blood sample is measured, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion. A red blood cell count per unit volume within the blood sample is determined by dividing the hematocrit measured within the first portion by the mean corpuscular volume measured within the second portion. Other applications are also described.

Performing optical measurements on a sample

Apparatus and methods are described including placing a first portion of a biological sample into a source sample portion chamber of a sample carrier, and placing a second portion of the biological sample into a diluted sample portion chamber. The second portion of the biological sample is diluted with respect to the first portion of the biological sample. Using an optical measurement device, a bulk-level measurand of the sample that relates to a parameter of the biological sample as a whole is measured, by performing a measurement upon the first portion of the biological sample. Microscopic images of the second portion of the biological sample are acquired. Other applications are also described.

Performing measurements on a sample

Apparatus and methods are described including placing a sample into a sample carrier that comprises a plurality of regions having upper and lower surfaces, having respective heights that are different from each other, and being configured such that cells form a monolayer, the monolayer within respective regions of the sample carrier having respective, different densities from each other, due to the respective regions of the sample carrier having respective heights that are different from each other. Microscopic images are acquired of each of the plurality of regions. Measurements are performed upon cell types that have a relatively high density upon microscopic images of a region of the sample chamber having a relatively low height, and measurements are performed upon cell types that have a relatively low density upon microscopic images of a region of the sample chamber having a relatively great height. Other applications are also described.

Sample carrier for optical measurements

Apparatus and methods are described for determining a property of a biological sample. A sample carrier () includes one or more sample chambers () that define at least a first region () and a second region (), the height of the one or more sample chambers varying between the first and second regions in a predefined manner. A computer processor () receives data relating to a first optical measurement that is performed upon a portion of the sample that is disposed within the first region, receives data relating to a second optical measurement that is performed upon a portion of the sample that is disposed within the second region, and determines the property of the sample by using a relationship between the first optical measurement, the second optical measurement, and the predefined variation in height between the regions. Other applications are also described.

Apparatus and methods are described for use with a blood sample (), including measuring hemoglobin concentration within at least a portion () of the blood sample, by performing a first measurement on the blood sample. Mean corpuscular hemoglobin in the blood sample () is measured, by performing a second measurement on the blood sample (). A parameter of the blood sample is determined, based on a relationship between the concentration of hemoglobin and the mean corpuscular hemoglobin. Other applications are also described.

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Rehovot, Israel

Amir Zait