National Research Council of Canada

Amine A Kamen

Yves Durocher

Sylvie Perret

Phuong Lan Pham

Bernard Massie

Anne Marcil

Chun Fang Shen

Julia Transfiguracion

Aziza Manceur

Wei Zou

Christine Gadoury

M T Johanne C&ocirc;t&eacute;

The present invention relates to hemagglutinin-specific antibodies, fragments thereof, and uses thereof. More specifically, these antibodies and fragments thereof are able to recognize antigen from multiple influenza strains.

Hemagglutinin-specific antibodies and uses thereof

Disclosed is a new process for the production of recombinant proteins, by transient transfection of suspension-grown human embryonic kidney cells (293 cell line and its genetic variants) with an expression vector, using polyethylenimine (PEI) as a transfection reagent. In a preferred embodiment, the process uses 293E cells expressing the Epstein-Barr virus (EBV) EBNA 1 protein, in combination with an oriP-based episomal expression vector having an improved cytomegalovirus expression cassette comprising the CMV5 promoter. The process combines in a single step the cell growth, transfection and protein expression, is carried out without changing the culture medium, and allows to achieve high expression levels in a short period of time. The process may be carried out in a serum-free, low-protein culture medium, is easily scalable, compatible with continuous production processes, and fully adapted to high-throughput production of milligram quantities of recombinant proteins.

Enhanced production of recombinant proteins by transient transfection of suspension-growing mammalian cells

A method of using a cell to produce virus is provided involving growing cells under hyperosmotic conditions during a growth phase of the cells, infecting or transfecting the cells grown under hyperosmotic conditions with a virus, and maintaining the infected or transfected cells under less stressful conditions during a production phase of the infected or transfected cells to produce more of the virus. Viral productivity is improved by this method.

Method of viral production in cells

In accordance with the present invention, there is provided a new method for detection and quantification of virus and viral particles, comprising the step of labeling the nucleic acids of an intact virus or viral particle with a dye that emits fluorescence once complexed with the nucleic acids and detecting the virus or viral particle with a chromatograph equipped with a fluorescence detector.

Detection of intact recombinant viruses

The present invention relates to a method of serum-free production of recombinant proteins and adenoviral vectors; cell lines viable in a serum-free medium and method of obtaining same.

Growing community of inventors

Montréal, Canada

Amine A Kamen