Centre National De La Recherche Scientifique

Biomerieux, Inc.

Universite Claude Bernard – Lyon1

Agence Francaise De Securite Sanitaire Des Aliments-afssa

Agence Francaise De Securite Sanitaire Des Aliments

Universite Claude Bernard Lyon and Biomerieux

Universite Claude Bernard—lyon

Ambroise Martin

Anthony William Coleman

Aly Moussa

Herve Perron

Eric Da Silva

Marilyne Dupin

Patrick Shahgaldian

Edwige Leclere

Hervé Perron

Frederic Ronzon

Frédéric Ronzon

Anna Bencsik-Reynier

Anna Benscik-Reynier

Adina Nicoleta Lazar

A process of concentration by precipitation of PrPfor detecting or diagnosing PrP, wherein a tissue or biological fluid stemming from or obtained from an animal or human organism is brought together with an antibiotic from the family of aminoglycosides, preferably streptomycin or one of its derivatives. The use of such an antibiotic for eliminating PrPfrom a tissue or biological fluid and a kit for diagnosing pathologies associated with the presence of PrPis also disclosed.

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The invention relates to a method for detecting PrP in a biological human or animal sample that may contain PrP. The inventive method is characterised in that it uses a molecule containing at least one positive charge and/or at least one osidic bond and a ligand other than a proteinic ligand selected from macrocyclic ligands and glycosaminoglycanes.

Method for detecting PrP using at least one positive charge and/or at least one glycosidic bond and a ligand other than a protein ligand

A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrP, the resulting suspension then being reacted with an anti-PrPantibody, and the presence of PrP is then detected.

Process for detecting PrP using a macrocyclic adjuvant ligand

Growing community of inventors

Charly, France

Ambroise Martin