Roche Molecular Systems, Inc.

Nokia Corporation

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Amar Gupta

Stephen Gordon Will

Veeraiah Bodepudi

Igor Kozlov

David H Gelfand

Randall K Saiki

Alison Tsan

Thomas W Myers

Fred Lawrence Reichert

Nancy J Schoenbrunner

Chi Fai Ho

Dieter Heindl

Keith Bauer

Roy A Bohenzky

Nicolas Newton

Prabhas Kejriwal

Randall Saiki

Hannes Kuchelmeister

Madhu Grandhi

Christopher D Newhouse

Ravi Bail Bhat

Alex Bachmutsky

Alexander Nierth

John Niemiec

Concordio Anacleto

Christian Wellner

Thomas B Myers

Laura Geyer

Janine Montiel

Kevin Janssen

Roy Bohenzky

The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

Methods for performing multiplexed real-time PCR

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Photocleavable nucleotide reagents with high stability

The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

Methods for performing multiplexed PCR

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Reversibly protected nucleotide reagents with high thermal stability

The present invention relates to methods and compositions for the stabilization of specific RNA molecules that can either be the target for detection or the control standard.

Generic method for the stabilization of specific RNA

The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.

Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch

The present invention provides for novel methods and compositions for nucleic acid sequence detection. Unique, identifying cleavage fragments from probes, bound to target nucleic acids, are produced during PCR by the 5'-nuclease activity of the polymerase. The identity of the targets can be determined by identifying the unique cleavage fragments.

Nucleic acid target identification by structure based probe cleavage

The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.

Methods for the enrichment of mutated nucleic acid from a mixture

Multiplexed nucleic acid target identification by strucutre based probe cleavage

The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes novel compounds and methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.

Compounds and methods for the enrichment of mutated nucleic acid from a mixture

The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2' position.

Sequencing and genotyping using reversibly terminating nucleotides

An improved method of preventing carryover contamination of an amplification reaction involves treating uracil-containing DNA with uracil-N-DNA glycosylase and heating the DNA in the presence of polyamines, such as spermidine, spermine and the like. Alternatively, after treatment with uracil-N-DNA glycosylase, the reaction is further incubated with an enzyme having AP lyase activity.

Method for the prevention of carryover contamination in nucleic acid amplification technologies

Growing community of inventors

Danville, CA, United States of America

Amar Gupta