City College of New York

Other

The City University of New York

Alvin Katz

Robert R Alfano

Yuanlong Yang

Scott A Alfano

Singaravelu Ganesan

Aleksandra Alimova

Yang Yuanlong

An apparatus and method for medical practitioners to detect the presence of abnormal cells including cancerous and pre-cancerous cells by using a transport capsule containing an imaging apparatus including UV sources and fluorescence detectors for obtaining images and fluorescence data of biological cells and tissue. The method includes the steps of scanning biological tissue using an ultra-violet (UV) source to obtain fluorescence data, transferring fluorescence data and/or images using a radio frequency (RF) or other suitable means to a personal computer (PC) system, analyzing the image and/or fluorescence data in the PC, identifying -tissues with precancerous and cancerous cells, and optionally determining their precise location, and assessing the accuracy of the calculated fluoroscopic images.

Micro-scale compact device for in vivo medical diagnosis combining optical imaging and point fluorescence spectroscopy

The present application is directed to methods for detecting cancerous, pre-cancerous and benign/normal tissue using phosphorescence or the combination of fluorescence and phosphorescence emission from the tissue excited in 280 to 340 nm range. In particular, the application describes methods using a ratio of intensity of fluorescence emission to intensity of phosphorescence emission to provide a rapid cancer, pre-cancer and benign detection method. Normal human breast and prostate tissue, excited in 270 to 300 nm range, express the emission signatures at 340 nm, 440 nm and 500 nm. Cancerous tissue shows only minor emissions at 500 nm, thus the ratio of 340 nm peak to 500 nm peak emission intensities provides information about malignancy of a tissue. Fiber optic based instrumentation can be used to collect the luminescence signal from an area of a tissue less than 1 mm in diameter and using the described method can be screened to determine the state of the tissue sample taken. The combination of fiber optics and 2D translation stage provides 2D maps of intensity's ratio of the tissue surface and aids in localizing the malignancy region of the tissue.

Phosphorescence and fluorescence spectroscopy for detection of cancer and pre-cancer from normal/benign regions

A method of detecting cancer or precancerous conditions in a tissue or cell using fluorescence excitation spectroscopy. According to one embodiment of the method, a tissue is tested for cancer or a precancerous condition by exciting the tissue with substantially monochromatic light at 268 nm and then at 289 nm, with the resultant native fluorescence emitted from the tissue following each excitation being measured. A ratio of the fluorescence intensities, e.g., I.sub.289 /I.sub.268, is then calculated. If I.sub.289 /I.sub.268 is greater than 1.5, the tissue is cancerous or precancerous whereas if I.sub.289 /I.sub.268 is less than 1.5, the tissue is not cancerous or precancerous.

Detection of cancer and precancerous conditions in tissues and/or cells

A method for detecting cancerous tissue using optical spectroscopy and Fourier analysis. According to a preferred embodiment, a tissue sample is illuminated with light at a wavelength of approximately 300 nm. Next, a fluorescence emission spectrum for the tissue sample is obtained by measuring the resultant fluorescence from the tissue sample over the spectral region from approximately 320 nm to approximately 580 nm. The phase and amplitude for the first three Fourier transform harmonics are then determined. The phase and amplitude determinations for one or more of the three harmonics are then compared to appropriate standards obtained from cancerous and non-cancerous tissue samples. The accuracy of the method can be improved further by comparing the phase and amplitude calculations for at least two harmonics.

Growing community of inventors

Bronx, NY, United States of America

Alvin Katz