Dnae Group Holdings Limited

Alpesh Patel

Samuel Reed

Christofer Toumazou

Ben Lane

Chad Schneider

John Paul Palmer-Felgate

David Whitaker

Zahid Ansari

Kurt Kolb

Hua Bai

Daniel Morley

Leila Shepherd

Daniel Coomber-Alford

Angel Chan-Ju Wang

Maurizio Lamura

Jessica Fisher

Panteleimon Athanasiou

Ginny Jorgensen

Krishna Amin

Alex K Flamm

Sam Reed

Alex Flamm

John Palmer-Felgate

Angel Wang

Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

Sensing apparatus for amplification and sequencing of template polynucleotides and array for amplification of template polynucleotides

A system, device, and method for receiving, preparing and identifying a biological sample. Some parts may be user held and actuated. The sample may be detected by an array of sensors. The system may comprise reusable and disposable components arranged to be connected together. The biological sample may contain nucleic acids, proteins, or other molecules to be detected.

Biosensor device and system

An apparatus for monitoring amplification of a nucleic acid by providing a pH sensor or pH indicator, a nucleic acid in an amplification mixture, and an isothermal amplification enzyme.

Apparatus for use in isothermal amplification

A plug side surface of a plug housing is provided with a claw portion. The claw portion includes a plug lock surface facing in a direction away from a connector mounting surface. Each assistant fitting of a receptacle connector includes a receptacle lock surface that faces in a direction approaching the connector mounting surface and is opposed to the plug lock surface in a mated state. The plug lock surface includes a lock maintaining surface and an unlocking surface. Assuming that an angle formed between a reference plane and the lock maintaining surface is a lock maintaining angle and an angle formed between the reference plane and the unlocking surface is an unlocking angle, the lock maintaining angle is smaller than the unlocking angle.

A method of monitoring amplification of a nucleic acid by providing a nucleic acid and an amplification mixture using the kit of isothermal reagents to a pH sensor or pH indicator, amplifying the nucleic acid using isothermal amplification, and detecting a change in pH due to the amplification using the pH sensor or pH indicator. The kit of reagents comprises a magnesium salt, a quaternary ammonium salt, and an alkali base.

Reaction mixture for use in isothermal amplification

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London, United Kingdom

Alpesh Patel